ELISA Kit for Stromal Cell Derived Factor 1 (SDF1)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 10x96T
ArtNr SEA122Hu-10
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Stromal Cell Derived Factor 1
Alternative Names
CXCL12, PBSF, P-BSF, SCYB12, SDF1-A, SDF1-B, SDF1A, SDF1B, TLSF-A, TLSF-B, TPAR1, Pre-B Cell Growth-Stimulating Factor, Chemokine C-X-C-Motif Ligand 12
Uniprot
P48061
Detection range
0.156-10ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<0.057ng/mL
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Stromal Cell Derived Factor 1 (SDF1).
No significant cross-reactivity or interference between Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stromal Cell Derived Factor 1 (SDF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stromal Cell Derived Factor 1 (SDF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stromal Cell Derived Factor 1 (SDF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stromal Cell Derived Factor 1 (SDF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Cytokine, Tumor immunity, Infection immunity,
References
Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD)
Elevated PLGF contributes to small-cell lung cancer brain metastasis
Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis
Serum levels of selected chemokines in systemic lupus erythematosus patients
The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trial
Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary men
Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression
Circulating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke Patients
The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders
Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells
Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder
A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural Effusion
A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity
High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cellsand depressed shedding of vascular endothelial cells undergoing hypoxia.
Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study.
Low?intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1?mediated SDF?1 expression

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Menge: 10x96T
Lieferbar: In stock
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