ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 10x96T
ArtNr SEA128Hu-10
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Tissue Inhibitors Of Metalloproteinase 2
Alternative Names
CSC-21K, TIMP Metallopeptidase Inhibitor 2
Uniprot
P16035
Detection range
0.94-60ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<0.41ng/mL
Sample type
serum, plasma, tissue homogenates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Tissue Inhibitors Of Metalloproteinase 2 (TIMP2).
No significant cross-reactivity or interference between Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 2 (TIMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 2 (TIMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Tumor immunity,
References
Bone morphogenetic protein-2: a potential regulator in scleral remodeling
A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches
Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course
Plasma inflammatory biomarkers for Huntington’s disease patients and mouse model
From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.
The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells
From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases

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Menge: 10x96T
Lieferbar: In stock
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