ELISA Kit for Secreted Frizzled Related Protein 5 (SFRP5)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 5x96T
ArtNr SEC842Hu-5
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Uniprot
Q5T4F7
Detection range
1.56-100ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Organism species
Homo sapiens (Human)
Sensitivity
The minimum detectable dose of this kit is typically less than 0.62ng/mL
Sample type
serum, plasma, tissue homogenates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 5 (SFRP5).
No significant cross-reactivity or interference between Secreted Frizzled Related Protein 5 (SFRP5) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secreted Frizzled Related Protein 5 (SFRP5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secreted Frizzled Related Protein 5 (SFRP5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 5 (SFRP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 5 (SFRP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 5 (SFRP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 5 (SFRP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Alternative Names
SARP3, Secreted Apoptosis Related Protein 3, Frizzled-related protein 1b
Item Name
Secreted Frizzled Related Protein 5
Research Area
Signal transduction,
Reference
Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance
Sfrp5 correlates with insulin resistance and oxidative stress
Balanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in children
SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in children
Low serum level of secreted frizzled-related protein 5, an anti-inflammatory adipokine, is associated with coronary artery disease
Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation
Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammation
Serum secreted frizzled?related protein 5 levels differentially decrease in patients with hepatitis B virus?associated chronic infection and hepatocellular carcinoma
Circulating SFRP5 levels are elevated in drug?na?ve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controls
The wingless-related integration site-5a/secreted frizzled-related protein-6 system is dysregulated in human sepsis
Circulating SFRP5 levels are elevated in drug-na
Prediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levels
Sfrp5 associates with beta-cell function in humans
Bone marrow sFRP5 level is negatively associated with bone formation markers
Plasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adults
Menge: 5x96T
Lieferbar: In stock
Listenpreis: 2.716,15 €
Preis: 2.716,15 €
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