ELISA Kit for Renalase (RNLS)

Hersteller Cloud-Clone
Kategorie Produkte / ELISA
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 5x96T
ArtNr SEC845Hu-5
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Uniprot
Q5VYX0
Detection range
3.12-200ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Organism species
Homo sapiens (Human)
Sensitivity
The minimum detectable dose of this kit is typically less than 1.37ng/mL
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Renalase (RNLS).
No significant cross-reactivity or interference between Renalase (RNLS) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renalase (RNLS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renalase (RNLS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Renalase (RNLS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Renalase (RNLS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Renalase (RNLS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Renalase (RNLS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Alternative Names
MAO-C, C10orf59, FAD-Dependent Amine Oxidase, Monoamine oxidase-C, alpha-NAD(P)H oxidase/anomerase
Item Name
Renalase
Research Area
Enzyme & Kinase,
Reference
Renalase, kidney function, and markers of endothelial dysfunction in renal transplant recipients
Renalase, Stroke, and Hypertension in Hemodialyzed Patients
Renalase in peritoneal dialysis patients is not related to blood pressure, but to dialysis vintage.
Vascular adhesion protein-1 and renalase in regard to diabetes in hemodialysis patients
Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients
VAP-1 in chronic kidney disease and diabetic kidney disease
The relationship between dopamine and renalase in type 2 diabetic patients with and without diabetic nephropathy
Renalase, a novel enzyme involved in blood pressure regulation, is related to kidney function but not to blood pressure in hemodialysis patients.
Renalase in hypertension and kidney disease
Markers of increased cardiovascular risk in patients with chronic kidney disease
Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients
Normal values for urine renalase excretion in children
Low plasma renalase concentration in hypertensive patients after surgical repair of coarctation of aorta
Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adults
Circulating renalase, catecholamines, and vascular adhesion
Renalase levels in children with solitary functioning kidney
SP321 RENALASE, RENAL FUNCTION AND BIOMARKERS OF ENDOTHELIAL DYSFUNCTION IN CHRONIC KIDNEY DISEASE PATIENTS
PP.27.04: ENDOTHELIAL DYSFUNCTION AND ARTERIAL HYPERTENSION IN THE WORKING POPULATION (MERIDIAN STUDY).
Renalase is Removed by Kidneys and During Dialysis – Excess Related to CKD Complications?
Serum Renalase Levels Correlate with Disease Activity in Lupus Nephritis
Plasma renalase concentration before and after radiofrequency renal denervation in patients with resistant hypertension
Urinary renalase concentration in patients with preserved kidney function undergoing coronary angiography.
PLASMA RENALASE CONCENTRATION BEFORE AND AFTER RADIOFREQUENCY RENAL DENERVATION IN PATIENTS WITH RESISTANT HYPERTENSION: PRELIMINARY RESULTS
Increased serum renalase in hemodialysis patients: is it related to left ventricular hypertrophy?
High-performance Liquid Chromatography Measured Metabolites of EndogenousCatecholamines and Their Relations to Chronic Kidney Disease and High Blood Pressure in Heart Transplant Recipients.
Online Haemodiafiltration Improves Inflammatory State in Dialysis Patients: A Longitudinal Study.
Renalase in Children with Glomerular Kidney Diseases
Plasma Renalase is Not Associated with Blood Pressure and Brachial-Ankle Pulse Wave Velocity in Chinese Adults With Normal Renal Function
Rs10887800 renalase gene polymorphism influences the level of circulating renalase in patients undergoing hemodialysis but not in healthy controls
Increased serum renalase in peritoneal dialysis patients: Is it related to cardiovascular disease risk
Menge: 5x96T
Lieferbar: In stock
Listenpreis: 2.218,31 €
Rabatt: -10%
Preis: 1.996,48 €
Sie sparen: 221,83 €
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