ELISA Kit for Folate Receptor 1, Adult (FOLR1)

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Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 10x96T
ArtNr SEF460Hu-10
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Folate Receptor 1, Adult
Alternative Names
FBP, FOLR, FR-alpha, MOv18, Adult folate-binding protein, KB cells FBP, Ovarian tumor-associated antigen MOv18
Uniprot
P15328
Detection range
15.6-1, 000pg/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<5.6pg/mL
Sample type
Serum, plasma, tissue homogenates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Folate Receptor 1, Adult (FOLR1).
No significant cross-reactivity or interference between Folate Receptor 1, Adult (FOLR1) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Folate Receptor 1, Adult (FOLR1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Folate Receptor 1, Adult (FOLR1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Folate Receptor 1, Adult (FOLR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Folate Receptor 1, Adult (FOLR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Folate Receptor 1, Adult (FOLR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Folate Receptor 1, Adult (FOLR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
CD & Adhesion molecule,
References
Association of folic acid receptor alpha in maternal serum with neural tube defects
Quantitation of rare circulating tumor cells by folate receptor alpha ligand-targeted PCR in bladder transitional cell carcinoma and its potential diagnostic significance.

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Menge: 10x96T
Lieferbar: In stock
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