ELISA Kit for Interleukin 4 (IL4)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 10x96T
ArtNr SEA077Mu-10
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Interleukin 4
Alternative Names
BSF1, BCGF1 , B Cell Stimulatory Factor 1, Lymphocyte stimulatory factor 1, B Cell Growth Factor, Binetrakin, Pitrakinra
Uniprot
P07750
Detection range
7.8-500pg/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<3.3pg/mL
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 4 (IL4).
No significant cross-reactivity or interference between Interleukin 4 (IL4) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 4 (IL4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 4 (IL4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 4 (IL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 4 (IL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 4 (IL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 4 (IL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Cytokine, Immune molecule,
References
Characterization of Anti-Inflammatory Properties and Evidence for No Sedation Liability for the Novel Antihistamine SUN-1334H
Protective effects of neferine on amiodarone-induced pulmonary fibrosis in mice
Roles of lipoxin A4 receptor activation and anti-interleukin-1beta antibody on the toll-like receptor 2/mycloid differentiation factor 88/nuclear factor-kappaB pathway in airway inflammation induced by ovalbumin
Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-??B in murine model of asthma
Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism
Recombinant Mip-PilE-FlaA dominant epitopes vaccine candidate against Legionella pneumophila.
Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathology
Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-kappaB in murine model of asthma
Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system.
Oral administration of Clostridium butyricum CGMCC0313?1 reduces ovalbumin?induced allergic airway inflammation in mice
Anti-asthmatic efect of pitavastatin through aerosol inhalation is associated with CD4+ CD25+ Foxp3+ T cells in an asthma mouse model
Sex differences in complex regional pain syndrome type I (CRPS-I) in mice
Commensal bacteria aggravate allergic asthma via NLRP3/IL-1beta signaling in post-weaning mice
Chitosan oligosaccharide ameliorates acute lung injury induced by blast injury through the DDAH1/ADMA pathway
Involvement of T-Helper 9 Activation in a Mouse Model of Allergic Rhinitis
Natural killer T cell ligand alpha-galactosylceramide protects against gut ischemia reperfusion-induced organ injury in mice
Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi')
ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma

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Menge: 10x96T
Lieferbar: In stock
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