ELISA Kit for Leptin (LEP)

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Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 10x96T
ArtNr SEA084Mu-10
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Leptin
Alternative Names
OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein
Uniprot
P41160
Detection range
0.156-10ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<0.054ng/mL
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Leptin (LEP).
No significant cross-reactivity or interference between Leptin (LEP) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leptin (LEP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leptin (LEP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leptin (LEP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leptin (LEP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leptin (LEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leptin (LEP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Metabolic pathway, Endocrinology, Cardiovascular biology,
References
Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet.
CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes
Oxytocin reverse ovariectomy-induced Osteoper and body fat gain
cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis
The leptin, dopamine and serotonin receptors in hypothalamic POMC-neurons of normal and obese rodents
The Effect of Aerobic Training on Tumor Growth, Adiponectin, Leptin and Ghrelin in Mice with Breast Cancer
Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 …
Effects of Metformin Combined with Lactoferrin on Lipid Accumulation and Metabolism in Mice Fed with High-Fat Diet
The evidence of metabolic-improving effect of metformin in Ay/a mice with genetically-induced melanocortin obesity and the contribution of hypothalamic …
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Menge: 10x96T
Lieferbar: In stock
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