ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 5x96T
ArtNr SEA128Mu-5
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Tissue Inhibitors Of Metalloproteinase 2
Alternative Names
CSC-21K, TIMP Metallopeptidase Inhibitor 2
Uniprot
P25785
Detection range
31.2-2, 000pg/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<12.7pg/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Tissue Inhibitors Of Metalloproteinase 2 (TIMP2).
No significant cross-reactivity or interference between Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 2 (TIMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 2 (TIMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Tumor immunity,
References
Oral Administration of 3, 3?-Diindolylmethane Inhibits Lung Metastasis of 4T1 Murine Mammary Carcinoma Cells in BALB/c Mice
Oral administration of benzyl-isothiocyanate inhibits solid tumor growth and lung metastasis of 4T1 murine mammary carcinoma cells in BALB/c mice
Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke
The Complementary Effects of Atorvastatin and Exercise Treatment on the Composition and Stability of the Atherosclerotic Plaques in ApoE Knockout Mice
Plasma inflammatory biomarkers for Huntington’s disease patients and mouse model

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Menge: 5x96T
Lieferbar: In stock
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