ELISA Kit for Terminal Complement Complex C5b-9 (C5b-9)

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Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 10x96T
ArtNr SEC350Mu-10
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Terminal Complement Complex C5b-9
Alternative Names
MAC, Membrane Attack Complex
Detection range
1.56-100ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<0.61ng/mL
Sample type
serum, plasma and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Terminal Complement Complex C5b-9 (C5b-9).
No significant cross-reactivity or interference between Terminal Complement Complex C5b-9 (C5b-9) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Terminal Complement Complex C5b-9 (C5b-9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Terminal Complement Complex C5b-9 (C5b-9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Terminal Complement Complex C5b-9 (C5b-9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Terminal Complement Complex C5b-9 (C5b-9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Terminal Complement Complex C5b-9 (C5b-9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Terminal Complement Complex C5b-9 (C5b-9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Signal transduction, Metabolic pathway, Apoptosis, Infection immunity,
References
Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis
The efficacy of recombinant human soluble thrombomodulin for the treatment of shiga toxin associated hemolytic uremic syndrome model mice
Adjuvant treatment with dexamethasone plus anti-C5 antibodies improves outcome of experimental pneumococcal meningitis: a randomized controlled trial
Thrombin?activatable fibrinolysis inhibitor influences disease severity in humans and mice with pneumococcal meningitis
Mannose-binding lectin-associated serine protease 2 (MASP-2) contributes to poor disease outcome in humans and mice with pneumococcal meningitis

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Menge: 10x96T
Lieferbar: In stock
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