ELISA Kit for Leptin (LEP)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Rat
Applikationen ELISA
Menge 48T
ArtNr SEA084Ra-48
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Leptin
Alternative Names
OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein
Uniprot
P50596
Detection range
0.312-20ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<0.129ng/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Leptin (LEP).
No significant cross-reactivity or interference between Leptin (LEP) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leptin (LEP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leptin (LEP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leptin (LEP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leptin (LEP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leptin (LEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leptin (LEP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Metabolic pathway, Endocrinology, Cardiovascular biology,
References
Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet
Effect of Argyreia speciosa root extract on cafeteria diet-induced obesity in rats
Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats
Maternal high-fat diet feeding during pregnancy and lactation augments lung inflammation and remodeling in the offspring
Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis
Tinospora crispa
Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines
The bromodomain inhibitor N-methyl pyrrolidone reduced fat accumulation in an ovariectomized rat model
Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats.
Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague …
Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21
Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats
??????? ?????????? ?? ?????????????? ?????????? ? ???????????????? ?????????? ??????? ? ???? ? ????????? …
Raspberry ketone and Garcinia Cambogia rebalanced disrupted insulin resistance and leptin signaling in rats fed high fat fructose diet
Obesity Aggravates Acute Pancreatitis via Damaging Intestinal Mucosal Barrier and Changing Microbiota Composition in Rats
Effect of High Intensity Interval Training (HIIT) on visceral and subcutaneous levels of leptin and plasma insulin and glucose in male Wistar rats
Anti-obesity effect of argel (Solenostemma argel) on obese rats fed a high fat diet
A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese …

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Menge: 48T
Lieferbar: In stock
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