ELISA Kit for Osteoprotegerin (OPG)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Rat
Applikationen ELISA
Menge 5x96T
ArtNr SEA108Ra-5
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Osteoprotegerin
Alternative Names
TNFRSF11B, TNFRSF11-B, OCIF, OPG, TR1, Tumor Necrosis Factor Receptor Superfamily Member 11b, Osteoclastogenesis Inhibitory Factor
Uniprot
O08727
Detection range
3.12-200pg/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<1.21pg/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Osteoprotegerin (OPG).
No significant cross-reactivity or interference between Osteoprotegerin (OPG) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteoprotegerin (OPG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteoprotegerin (OPG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteoprotegerin (OPG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteoprotegerin (OPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteoprotegerin (OPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osteoprotegerin (OPG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Cytokine, Metabolic pathway, Bone metabolism,
References
Protective Effect of Amlodipine on Rat Bone Tissue after Orchidectomy
Protective effect of atorvastatin on bone tissue in orchidectomised male albino Wistar rats
The effects of chronic zoledronate usage on the jaw and long bones evaluated using RANKL and osteoprotegerin levels in an animal model
Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cells
The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism.
Effect of Nicotine on RANKL and OPG and Bone Mineral Density
Carvedilol promotes neurological function, reduces bone loss and attenuates cell damage after acute spinal cord injury in rats
A novel model of bisphosphonate-related osteonecrosis of the jaw in rats
Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy
Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/beta-catenin pathway
The effects of strength training and raloxifene on bone health in aging ovariectomized rats
Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats.
Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats.
Skeletal Site-specific Effects of Zoledronate on in vivo Bone Remodeling and in vitro BMSCs Osteogenic Activity.
Assessment of bone metabolism and biomechanical properties of the femur, following treatment with anastrozole and letrozole in an experimental model of menopause
A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/ÃŽA²-catenin Signaling Pathways in Ovariectomized Rats
Peroxisome proliferator?activated receptor gamma plays dual roles on experimental periodontitis in rats
Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats

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Menge: 5x96T
Lieferbar: In stock
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