ELISA Kit for Cubilin (CUBN)

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Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Rat
Applikationen ELISA
Menge 5x96T
ArtNr SEC411Ra-5
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Cubilin
Alternative Names
IFCR, MGA1, gp280, Intrinsic Factor Cobalamin Receptor, 460 kDa receptor, Intestinal intrinsic factor receptor, Intrinsic factor-vitamin B12 receptor
Uniprot
O70244
Detection range
0.312-20ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<0.123ng/mL
Sample type
Tissue homogenates, cell lysates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Cubilin (CUBN).
No significant cross-reactivity or interference between Cubilin (CUBN) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cubilin (CUBN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cubilin (CUBN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cubilin (CUBN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cubilin (CUBN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cubilin (CUBN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cubilin (CUBN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Signal transduction, Metabolic pathway, Nutrition metabolism, Dermatology,

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Menge: 5x96T
Lieferbar: In stock
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