TP53 Monoclonal Antibody

ELISA, WB; Recommended dilution: WB:1:500-1:5000

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Purified recombinant human p53 protein fragments expressed in E.coli.

Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Cell Biology

Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Purified recombinant human p53 protein fragments expressed in E.coli.