Phospho-RAF1 (Ser642) Antibody

Polyclonal Antibody

RAF1

c Raf antibody, C-raf antibody, C-Raf proto-oncogene, serine/threonine kinase antibody, CMD1NN antibody, Craf 1 transforming gene antibody, cRaf antibody, Craf1 transforming gene antibody, EC 2.7.11.1 antibody, kinase Raf1 antibody, Murine sarcoma 3611 oncogene 1 antibody, NS5 antibody, Oncogene MIL antibody, Oncogene RAF1 antibody, OTTHUMP00000160218 antibody, OTTHUMP00000207813 antibody, OTTHUMP00000209389 antibody, Protein kinase raf 1 antibody, Proto-oncogene c-RAF antibody, Raf 1 antibody, Raf 1 proto oncogene serine/threonine kinase antibody, RAF antibody, Raf proto oncogene serine/threonine protein kinase antibody, RAF proto-oncogene serine/threonine-protein kinase antibody, RAF-1 antibody, RAF1 antibody, RAF1_HUMAN antibody, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody, v raf 1 murine leukemia viral oncogene homolog 1 antibody, v-raf murine sarcoma viral oncogene homolog 1 antibody, v-raf-1 murine leukemia viral oncogene-like protein 1 antibody, vraf1 murine leukemia viral oncogene homolog 1 antibody

Peptide sequence around phosphorylation site of Serine 642(T-T-S(p)-P-R) derived from Human C-RAF.

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Bonner T.I., Nucleic Acids Res. 14:1009-1015(1986).
Ota T., Nat. Genet. 36:40-45(2004).
Mural R.J., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Signal Transduction

Peptide sequence around phosphorylation site of Serine 642(T-T-S(p)-P-R) derived from Human C-RAF.