CHEK1 (Ab-317) Antibody

Polyclonal Antibody

CHEK1

C85740 antibody, Cell cycle checkpoint kinase antibody, Checkpoint, S. pombe, homolog of, 1 antibody, Checkpoint kinase 1 antibody, Checkpoint kinase 1 homolog (S. pombe) antibody, CHEK 1 antibody, Chek1 antibody, Chk 1 antibody, Chk1 antibody, CHK1 checkpoint homolog (S. pombe) antibody, CHK1_HUMAN antibody, EC 2.7.11.1 antibody, rad27 antibody, Serine/threonine protein kinase Chk1 antibody, Serine/threonine-protein kinase CHK1 antibody, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody

Peptide sequence around aa.315ca.319 (S-S-S-Q-P) derived from Human Chk1.

Chek1 is a protein kinase that inhibits mitotic entry after DNA damage, required for the DNA damage checkpoint and is strongly similar to murine Chek1. Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The mouse and human proteins share 90% sequence identity through the protein kinase domains. The sequence of the 476-amino acid human Chek1 protein is 29%, 40%, and 44% identical to those of the fission yeast Chek1, C. elegans Chek1, and Drosophila 'grapes' (Grp) proteins, respectively. Chek1 is expressed ubiquitously as an approximately 2.4-kb mRNA, with the most abundant expression in thymus, testis, small intestine, and colon. The protein has altered mobility when isolated from cells treated with ionizing radiation, indicating that Chek1 is modified in response to DNA damage. In vitro, Chek1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, Chek1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-Cyclin-B complex and mitotic entry

Zhang YW, et al. (2005) Mol Cell, 19(5): 607-18.
Bhoumik A, et al. (2005) Mol Cell, 18(5): 577-87.
Rocha S, et al. (2005) EMBO J.
Clarke CA, et al. (2005) Biochem J.
Yu X, et al. (2004) Mol Cell Biol, 24(21): 9478-86.

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Epigenetics and Nuclear Signaling

Peptide sequence around aa.315~319 (S-S-S-Q-P) derived from Human Chk1.