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Human IL-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human IL-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

ArtNr	BOS-EZ0397
Hersteller	Boster
Menge	5 plates/kit
Quantity options	5 plates/kit
Kategorie	Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<1 pg/ml
Sequence	Belongs to the IL-2 family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Interleukin-2,IL-2,T-cell growth factor,TCGF,Aldesleukin,IL2
Lieferbar
Manufacturer - Category	EZ-Set™ ELISA Kits (Ab Pairs)
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	17628 MW
Gene Name	IL2
Gene Full Name	Interleukin-2
Manufacturer - Specificity	Natural and recombinant human IL-2
Background	Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: A21-T153
Protein Name	Interleukin-2
Protein Function	Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.
Subcellular Localization	Secreted.
Description	Human IL-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Sequence Similarities	Belongs to the IL-2 family.
Sample Data	Concentration (pg/ml) \| 0 \| 15.6 \| 31.2 \| 62.5 \| 125 \| 250 \| 500 \| 1000 O.D. \| 0.030 \| 0.123 \| 0.212 \| 0.336 \| 0.609 \| 1.031 \| 1.798 \| 2.197
Kit Components	Catalog number \| Description \| Quantity \| Storage of opened/reconstituted material EZ0397-CA \| Mouse anti- human IL2 monoclonal antibody (Capture Antibody) \| 500 µ l, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0397-DA \| Biotinylated goat anti- human IL2 polyclonal antibody (Detection Antibody) \| 500 µ l, 8.6 µ g/mL (recommended dilution 1:100) AR1103 \| Avidin-Biotin-Peroxidase Complex (ABC) \| 500 µ l (recommended dilution 1:100) EK0397-ST \| Lyophilized recombinant human IL2 standard \| 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.
Anticoagulant	heparin, EDTA or citrate
Material Required But Not Provided	1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T). Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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BOS-EZ0397-datasheet.pdf

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