beta-Defensin 3 (BD-3), Human

Hersteller GenScript
Kategorie
Typ Proteins
Specific against Human
Menge 1.0mg(2x500 ug)
ArtNr Z02756-1
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Product Line
Cytokine, Chemokines & Growth Factors
Description
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The ª-defensins are distinguished from the ß-defensins by the pairing of their three disulfide bonds. To date, four human ß-defensins have been identified, BD-1, BD-2, BD-3 and BD-4. ß-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The ß-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. ß-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. ß-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues.
M.W.
Approximately 5.1 KDa, a single non-glycosylated polypeptide chain containing 45 amino acids.
Purity
>98% by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 0.2EU/ug of rHuBD-3 as determined by LAL method.
Specific Activity
Fully biologically active when compared to standard. The ED50 determined by antimicrobial activity against E.coli. is less than 30000 ng/ml, corresponding to a specific activity of >, 33.3 IU/mg.
Storage
This lyophilized preparation is stable at 2-8 C, but should be kept at -20 C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 C to -70 C. Avoid repeated freeze/thaw cycles.
Formulation
Lyophilized from a 0.2µ, m filtered concentrated, solution in 20mM PB, pH 7.4, 130mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °, C. Further dilutions should be made in appropriate buffered solutions.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Sequence
GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK
Menge: 1.0mg(2x500 ug)
Lieferbar: In stock
Listenpreis: 2.695,55 €
Preis: 2.695,55 €
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