Epidermal Growth Factor

Der Epidermale Wachstumsfaktor (EGF) ist ein Protein, welches das Wachstum verschiedener epidermaler und epithelialer Gewebe stimuliert 1. Er besteht aus 53 Aminosäuren (6 kDa), welche über drei intramolekulare Disulfidbrückenbindungen verbunden sind 2. Er tritt verstärkt bei der Einleitung der Mitose auf. Zu Forschungszwecken kann er sowohl in vitro als auch in vivo verwendet werden. In machen Fibroblasten stimuliert er das beschriebene Wachstum auch in Zellkultur 1.

 

Epidermaler Wachstumsfaktor (EGFR) - Aminosäurensequenz

Abb Nr. 1: Aminosäuresequenz des epidermalen Wachstumsfaktors (EGF).

 

Epidermaler-Wachstumsfaktor-Rezeptor

EGF ist einer der wichtigsten Liganden der Epidermalen-Wachstumsfaktor-Rezeptorfamilie (EGFR). Der Rezeptor gehört zu Klasse der Transmembran-Glykoproteine und besteht aus einer extrazellulären Rezeptor-Domäne, einer transmembranen Region und einer intrazellulären Domäne, welche als Tyrosinkinase fungiert 3. Bindet z.B. EGF an EGFR, so findet eine Autophosphorylierung der Rezeptor Tyrosinkinase satt, was eine Aktivierung bestimmter Signaltransduktionswege zur Folge hat. Über diese Art der Signaltransduktion, werden unter anderem die Zellproliferation und die Zelldifferenzierung reguliert 4.

 

Epidermaler Wachstumsfaktor-Rezeptor (EGFR)

Abb. Nr. 2: EGFR besteht aus einer extrazellulären Rezeptor-Domäne, einer transmembranen Region und einer intrazellulären Domäne, welche als Tyrosinkinase fungiert.

Forschungsbereiche

EGFR spielt eine besondere Rolle bei der Erforschung von Krebserkrankungen, da er in einer Vielzahl von Tumorzellen überexprimiert wird. Eine Überexprimierung von EGFR konnte besonders in soliden Tumoren wie z.B. Brustkrebs, Kopf- und Nackenkrebs, nicht-kleinzelliger Lungenkrebs (NSCLC), Nierenkrebs, Eierstockkrebs und Darmkrebs gezeigt werden 5. Die Aktivierung des Rezeptors wird ebenfalls mit der Resistenz gegenüber Chemotherapie und Bestrahlung in Verbindung gebracht.

Die beschriebenen Eigenschaften von EGFR machen den Rezeptor zu einem wichtigen therapeutischen Ziel bei der Krebsbehandlung, da Wechselwirkungen mit EGFR zu einer Unterbrechung der Signaltransduktion führen können 6. Als Inhibition von EGFR konnten besonders monoklonale Antikörper, welche gegen die extrazelluläre Rezeptordomäne gerichtet sind und niedermolekulare Verbindungen, welche die intrazelluläre EGFR-Tyronsinkinase-Aktivität stören, als effektive beschrieben werden 7. Sowohl monoklonale Antikörper als auch niedermolekulare Verbindungen können dazu führen, dass die Zellproliferation unterbrochen wird und die Apotose erleichtert ist. Folglich können den genannten Substanzen sowohl zytotoxische als auch zytostatische Eigenschaften zugesprochen werden 7.

 

Epidermale Wachstumsfaktor (EGFR) Signalweg

Abb. Nr. 3: Die Abbildung zeigt eine ausführliche Übersicht des EGFR Signalwegs.

 

 

 

Produkte

Antikörper

RayBiotech Inc.

NameTypeSpecific againstHostArtNr.Menge

Rabbit Anti-­EGF Receptor

Antibody

Rabbit

other

127-10497

100ul

Mouse Anti-­Human EGF

Antibody

Mouse

Hu

128-10066-1

500 ug

Mouse Anti-­Human EGF

Antibody

Mouse

Hu

128-10066-2

1mg

Goat Anti-­Mouse EGF

Antibody

Goat

Mu

DS-PB-00691

0.1 mg

Goat Anti-­Mouse EGF [+Biotin]

Antibody

Goat

Mu

DS-PB-00692

50 ug

Rabbit Anti-­Human EGF

Antibody

Rabbit

Hu

DS-PB-00693

0.1 mg

Rabbit Anti-­Human EGF [+Biotin]

Antibody

Rabbit

Hu

DS-PB-00694

50 ug

Mouse Anti-­EGFR

Antibody

Mouse

other

MD-02-0003

100 ug

Mouse Anti-­EGFR

Antibody

Mouse

other

MD-02-0004

100 ug

Mouse Anti-­Human EGFR

Antibody

Mouse

Hu

MD-02-0005

100 ug

 

Cloud-Clone Corp.

NameTypeSpecific againstHostArtNr.Menge

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Hu

Mouse

MAA560Hu22_100ug

100ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Po

Mouse

MAA560Po21_100ug

100ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Ra

Mouse

MAA560Ra21_100ug

100ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Hu

Mouse

MAA560Hu22_20ug

20ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Po

Mouse

MAA560Po21_20ug

20ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Ra

Mouse

MAA560Ra21_20ug

20ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Po

Mouse

MAA560Po21_200ug

200ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Ra

Mouse

MAA560Ra21_200ug

200ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Hu

Mouse

MAA560Hu22_200ug

200ug

Monoclonal Antibody to Epidermal Growth Factor Receptor 2 (EGFR2)

Antibody Monoclonal

Hu

Mouse

MAB867Hu22_200ug

200ug

Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR)

Antibody Monoclonal

Hu

Mouse

MAA757Hu22_200ug

200ug

 

Sino Biological Inc.

NameTypeSpecific againstHostArtNr.Menge

Epidermal Growth Factor/­EGF Antibody, Rabbit PAb, Antigen Affinity Purified

Antibody

other

 

101619-T32-50

50 ug

Epidermal Growth Factor/­EGF Antibody, Rabbit PAb, Antigen Affinity Purified

Antibody

other

 

101619-T32-100

100 ug

Epidermal Growth Factor/­EGF Antibody, Rabbit PAb, Antigen Affinity Purified

Antibody

other

 

101619-T32-200

200 ug

Epidermal Growth Factor /­ EGF Antibody, Mouse MAb

Antibody

other

 

80446-MM02-50

50 ug

Epidermal Growth Factor /­ EGF Antibody, Mouse MAb

Antibody

other

 

80446-MM02-100

100 ug

Epidermal Growth Factor /­ EGF Antibody, Mouse MAb

Antibody

other

 

80446-MM02-200

200 ug

EGFR /­ HER1 Antibody, Rabbit MAb

Antibody

other

 

10001-RE11-50

50 ug

EGFR /­ HER1 Antibody, Rabbit MAb

Antibody

other

 

10001-RE11-100

100 ug

EGFR /­ HER1 Antibody, Rabbit MAb

Antibody

other

 

10001-RE11-1

1 mg

EGFR /­ HER1 /­ ErbB1 Antibody (APC), Mouse MAb

Antibody

other

 

10001-MM08-A-25

25 Tests

EGFR /­ HER1 /­ ErbB1 Antibody (APC), Rabbit MAb

Antibody

other

 

10001-RE01-A-25

25 Tests

EGFR /­ HER1 /­ ErbB1 Antibody (PE), Mouse MAb

Antibody

other

 

10001-MM08-P-25

25 Tests

EGFR /­ HER1 /­ ErbB1 Antibody (PE), Rabbit MAb

Antibody

other

 

10001-RE01-P-25

25 Tests

EGFR /­ HER1 /­ ErbB1 Antibody (FITC), Mouse MAb

Antibody

other

 

10001-MM08-F-25

25 Tests

EGFR /­ HER1 /­ ErbB1 Antibody (FITC), Rabbit MAb

Antibody

other

 

10001-RE01-F-25

25 Tests

EGFR /­ HER1 /­ ErbB1 Antibody, Rabbit MAb

Antibody

other

 

10001-R021-1

1 mg

 

ELISA-Kit

RayBiotech Inc.

NameTypeSpecific againstHostArtNr.Menge

Human EGF ELISA

Elisa-Kit

Human

E.coli

ELH-EGF-1

1 x 96-Well Strip Microplate Kit

Human EGF ELISA

Elisa-Kit

Human

E.coli

ELH-EGF-2

2 x 96-Well Strip Microplate Kit

Human EGF ELISA

Elisa-Kit

Human

E.coli

ELH-EGF-5

5 x 96-Well Strip Microplate Kit

Mouse EGF ELISA

Elisa-Kit

Mouse

E.coli

ELM-EGF-1

1 x 96-Well Strip Microplate Kit

Mouse EGF ELISA

Elisa-Kit

Mouse

E.coli

ELM-EGF-2

2 x 96-Well Strip Microplate Kit

Mouse EGF ELISA

Elisa-Kit

Mouse

E.coli

ELM-EGF-5

5 x 96-Well Strip Microplate Kit

Rhesus Monkey EGF ELISA

Elisa-Kit

Rhesus Monkey

 

ELK-EGF-1

1 x 96-Well Strip Microplate Kit

Rhesus Monkey EGF ELISA

Elisa-Kit

Rhesus Monkey

 

ELK-EGF-2

2 x 96-Well Strip Microplate Kit

Rhesus Monkey EGF ELISA

Elisa-Kit

Rhesus Monkey

 

ELK-EGF-5

5 x 96-Well Strip Microplate Kit

Custom Human EGF SpeedELISA

Elisa-Kit

Human

 

ELHS-EGF-1

1 x 96-Well Strip Microplate Kit

Custom Human EGF SpeedELISA

Elisa-Kit

Human

 

ELHS-EGF-2

2 x 96-Well Strip Microplate Kit

Custom Human EGF SpeedELISA

Elisa-Kit

Human

 

ELHS-EGF-5

5 x 96-Well Strip Microplate Kit

Rat EGF ELISA

Elisa-Kit

Rat

E.coli

ELR-EGF-1

1 x 96-Well Strip Microplate Kit

 

Sino Biological Inc.

NameTypeSpecific againstHostArtNr.Menge

Human EGFR /­ HER1 /­ ErbB1 ELISA Kit

Elisa-Kit

Human

Hu

KIT10001

1 Kit (96 Tests)

Human EGFR /­ HER1 /­ ErbB1 ELISA Kit

Elisa-Kit

Human

Hu

KIT10001-1

96 Tests

Cloud-Clone Corp.

NameTypeSpecific againstHostArtNr.Menge

Mini Samples ELISA Kit for Epidermal Growth Factor (EGF)

Elisa-Kit

other

MEA560Mu

96T

ELISA Kit for Epidermal Growth Factor (EGF)

Elisa-Kit

Hu

SEA560Hu

96T

ELISA Kit for Epidermal Growth Factor (EGF)

Elisa-Kit

Mu

SEA560Mu

96T

ELISA Kit for Epidermal Growth Factor (EGF)

Elisa-Kit

Po

SEA560Po

96T

ELISA Kit for Epidermal Growth Factor (EGF)

Elisa-Kit

Ra

SEA560Ra

96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Ra

SEB286Ra

96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Hu

SEB286Hu-5

5x96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Mu

SEB286Mu-5

5x96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Ra

SEB286Ra-5

5x96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Hu

SEB286Hu-10

10x96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Mu

SEB286Mu-10

10x96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Ra

SEB286Ra-10

10x96T

 

Proteine

RayBiotech Inc.

NameTypeSpecific againstHostArtNr.Menge

Human EGF, HEK293 Lysate

Proteins

other

 

230-10053-100

100 ug

Human EGF, HEK293 Transfected Cell Culture Supernatant

Proteins

other

 

230-20031-200

200 ul

Native Rat EGF

Proteins

Ra

 

DS-01-0091

10 ug

Human EGF

Proteins

Hu

 

213-10068-2

500 ug

EGF, Animal Free, Recombinant Mouse

Proteins

Mu

 

213-10069-AF-1

100 ug

EGF, Animal Free, Recombinant Mouse

Proteins

Mu

 

213-10069-AF-2

500 ug

Native Mouse EGF

Proteins

Mu

 

228-10361-2

50 ug

Native Rat EGF

Proteins

Ra

 

228-10363-2

5 ug

Native Rat EGF

Proteins

Ra

 

228-10363-3

10 ug

Neuregulin-­1/­Heregulin Alpha (EGF Domain), Human Recombinant

Proteins

Hu

 

228-20231-2

20 ug

Neuregulin-­1/­Heregulin Alpha (EGF Domain), Human Recombinant

Proteins

Hu

 

228-20231-3

1 mg

Proheparin-­Binding EGF-­like Growth Factor, Rat Recombinant

Proteins

Ra

 

228-20114-2

50 ug

Proheparin-­Binding EGF-­like Growth Factor, Rat Recombinant

Proteins

Ra

 

228-20114-3

1 mg

Recombinant Rat EGF

Proteins

Ra

 

229-20329

100 ug

Recombinant Human EGF

Proteins

Hu

 

229-20136

500 ug

Epidermal Growth Factor (EGF), Human recombinant

Proteins

Hu

 

268-10048-1

100 ug

Epidermal Growth Factor (EGF), Human recombinant

Proteins

Hu

 

268-10048-2

500 ug

Epidermal Growth Factor (EGF), Human recombinant

Proteins

Hu

 

268-10048-3

1 mg

Epidermal Growth Factor (EGF), Human recombinant

Proteins

Hu

 

268-10048-4

5 mg

EGF, Mouse recombinant

Proteins

Mu

 

268-10049-1

100 ug

EGF, Mouse recombinant

Proteins

Mu

 

268-10049-2

500 ug

EGF, Mouse recombinant

Proteins

Mu

 

268-10049-3

1 mg

EGF, Mouse recombinant

Proteins

Mu

 

268-10049-4

5 mg

EGF, Rat recombinant

Proteins

Ra

 

268-10050-1

20 ug

EGF, Rat recombinant

Proteins

Ra

 

268-10050-2

100 ug

 

Sino Biological Inc.

NameTypeSpecific againstHostArtNr.Menge

Human EGF /­ Epidermal Growth Factor Protein

Proteins

Hu

E.coli

10605-HNAE-1

1 mg

Human EGF /­ Epidermal Growth Factor Protein

Proteins

Hu

E.coli

10605-HNAE-500

500 ug

Human EGF /­ Epidermal Growth Factor Protein

Proteins

Hu

E.coli

10605-HNAE-100

100 ug

Mouse EGF /­ Epidermal Growth Factor Protein

Proteins

Mu

Human

50482-MNCH-1

1 mg

Mouse EGF /­ Epidermal Growth Factor Protein

Proteins

Mu

Human

50482-MNCH-500

500 ug

Mouse EGF /­ Epidermal Growth Factor Protein

Proteins

Mu

Human

50482-MNCH-100

100 ug

Human EGF /­ Epidermal Growth Factor Protein (Fc Tag)

Proteins

Hu

Human

10605-H01H-1

1 mg

Human EGF /­ Epidermal Growth Factor Protein (Fc Tag)

Proteins

Hu

Human

10605-H01H-500

500 ug

Human EGF /­ Epidermal Growth Factor Protein (Fc Tag)

Proteins

Hu

Human

10605-H01H-100

100 ug

Human NRG1-­beta 1 Protein (EGF Domain, Fc Tag)

Proteins

Hu

Human

11609-H01H-100

100 ug

Human NRG1-­beta 1 Protein (EGF Domain, Fc Tag)

Proteins

Hu

Human

11609-H01H-50

50 ug

Human NRG1-­beta 1 Protein (EGF Domain, Fc Tag)

Proteins

Hu

Human

11609-H01H-5

5 ug

Human NRG1-­alpha Protein (EGF Domain, Fc Tag)

Proteins

Hu

Human

13499-H01H-50

50 ug

Human NRG1-­alpha Protein (EGF Domain, Fc Tag)

Proteins

Hu

Human

13499-H01H-100

100 ug

Canine NRG1-­alpha Protein (EGF Domain, Fc Tag)

Proteins

Ca

Human

70084-D04H-5

5 ug

Canine NRG1-­alpha Protein (EGF Domain, Fc Tag)

Proteins

Ca

Human

70084-D04H-100

100 ug

Canine NRG1-­alpha Protein (EGF Domain, Fc Tag)

Proteins

Ca

Human

70084-D04H-50

50 ug

Canine NRG1-­alpha Protein (EGF Domain, Fc Tag)

Proteins

Ca

Human

70084-D04H-10

10 ug

Mouse EpCAM /­ TROP-­1 /­ TACSTD1 Protein (His Tag)

Proteins

Mu

Human

50591-M08H-100

100 ug

Mouse EpCAM /­ TROP-­1 /­ TACSTD1 Protein (His Tag)

Proteins

Mu

Human

50591-M08H-50

50 ug

Human EpCAM /­ TROP-­1 /­ TACSTD1 Protein (Fc Tag)

Proteins

Hu

Human

10694-H02H-100

100 ug

Human EpCAM /­ TROP-­1 /­ TACSTD1 Protein (His Tag)

Proteins

Hu

Human

10694-H08H-100

100 ug

Human EpCAM /­ TROP-­1 /­ TACSTD1 Protein (His Tag)

Proteins

Hu

Human

10694-H08H-50

50 ug

Human EpCAM /­ TROP-­1 /­ TACSTD1 Protein (Fc Tag)

Proteins

Hu

Human

10694-H02H-50

50 ug

Human TROP2 /­ TACSTD2 Protein (His & Fc Tag)

Proteins

Hu

Human

10428-H03H-200

200 ug

 

Literatur

1

Nair P. (2005) Epidermal growth factor receptor family and its role in cancer progression. Curr. Sci. 88, 890-898.

 

2

Carpenter G, Cohen S (May 1990). "Epidermal growth factor". The Journal of Biological Chemistry. 265(14): 7709–12. PMID 2186024

 

3

Carpenter G, Cohen S. Epidermal growth factor. Annu Rev Biochem 1979; 48:193–216.

 

4

Franklin WA, Veve R, Hirsch FR, et al. Epidermal growth factor receptor family in lung cancer and premalignancy. Semin Oncol 2002; 29 (Suppl 4):3–14.

 

5

Herbst RS, Langer CJ. Epidermal growth factor receptors as a target for cancer treatment: The emerging role of IMC-C225 in the treatment of lung and head and neck cancer. Semin Oncol 2002;29:27–36.

 

6

Iliakis G. Cell cycle regulation in irradiated and nonirradiated cells. Semin Oncol 1997; 24:602–615.

 

7

Wakeling AE, Barker AJ, Daview DH, et al. Specific inhibition of epidermal growth factor receptor tyrosine kinase by 4-anilinoquinazolines. Breast Cancer Res Treat 1996;38:67– 73

 

8

Wakeling AE, Barker AJ, Daview DH, et al. Specific inhibition of epidermal growth factor receptor tyrosine kinase by 4-anilinoquinazolines. Breast Cancer Res Treat 1996;38:67– 73