HIV-1 gp120/ Glycoprotein 120 ELISA Kit

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Hersteller Novateinbio
Kategorie
Typ Elisa-Kit
Specific against other
Menge 4x96T
ArtNr NS-E10006-4
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Background
Envelope glycoprotein GP120 (or gp120) is a glycoprotein exposed on the surface of the HIV envelope. Its main function is to bind to CD4 in human cells. The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein, GP 160, is proteolytically cleaved into GP 120 and gp41, which remain noncovalently associated with one another. GP 120 is one of the proteins that form the envelope of HIV. GP 120 projects from the surface of HIV and binds to the CD4+ molecule on helper T cells. GP 120 has been a logical experimental HIV vaccine because the outer envelope is the first part of the virus that encounters antibody.
Range
46.88 - 3000 pg/ml
Assay principle
This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA).
Storage
Store at 2 - 8C. Keep Standard and detection antibody at -20C.
Applications info
For quantitative determination of HIV-1 gp120 /Glycoprotein 120.
Sample Type
Culture Supernates, Serum, plasma, body fluids.
Detection Method
Chromogenic
Kit Components
1. Recombinant HIV-GP120 standard: 3ng/Vial -2.

2. One 96-well plate precoated with anti-HIV-GP120 antibody.

3. Sample diluent buffer: 10 mL

4. HRP conjugated anti-HIV-gp120 Ab: 10 uL, dilution 1:1000.

5. Detection Antibody diluent buffer: 12ml.

6. Assay diluent buffer: 10 ml.

7. TMB color developing agent A: 6 ml.

8. TMB color developing agent B: 6 ml.

9. TMB stop solution: 6ml.
Sensitivity
20 pg/ml
Expiration
Six months at 2 - 8 ?C.
Precision
Intro assay: CV : 5.4%

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Menge: 4x96T
Lieferbar: Out of stock
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