Anti-FTO Antibody

ArtNr	BOS-A00219
Hersteller	Boster
Menge	0.1 mg
Quantity options	0.1 mg 100 ul 100 ul
Kategorie	Metabolism Diabetes Glucose Homeostasis Obesity Immunohistochemistry Western Blot ELISA Immunofluorescence Immunostaining
Format	Liquid
Applikationen	WB, IF, ELISA, IHC-P
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Alias	ALKBH9,KIAA1752,Alpha-ketoglutarate-dependent dioxygenase FTO,Fat mass and obesity-associated protein
Lieferbar
Manufacturer - Category	Primary Antibodies
Storage Conditions	FTO antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	58282 MW
Clonality	Polyclonal
Application Details	FTO antibody can be used for detection of FTO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Gene Name	FTO
Gene Full Name	Fat mass and obesity associated
Background	Rising obesity rates are rapidly becoming a growing health concern in the developing world. The fat mass and obesity associated gene (FTO) is the first gene discovered to contribute to common forms of human obesity. FTO is a member of the non-heme dioxygenase superfamily, encoding a 2-oxoglutarate-dependent nucleic acid demethylase whose mRNA is widely expressed, especially in neurons of feeding-related nuclei of the brain. FTO mRNA in the arcuate nucleus in mice is up-regulated by feeding and down-regulated during fasting, although the opposite pattern has been observed in rats. At least four isoforms of FTO are known to exist.
Immunogen	FTO antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FTO. The immunogen is located within the first 50 amino acids of FTO.
Contents	FTO Antibody is supplied in PBS containing 0.02% sodium azide.
Purification	FTO Antibody is affinity chromatography purified via peptide column.
Concentration	1 mg/mL
Manufacturer - Research Category	Obesity
Protein Name	Fat mass and obesity associated
Protein Function	Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed:18775698, PubMed:20376003). Specifically demethylates N (6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103). Has no activity towards 1- methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation (PubMed:18775698, PubMed:20376003). In particular, it is involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:26287746).
Subcellular Localization	Nucleus
Description	Boster Bio Anti-FTO Antibody (Catalog # A00219). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Tissue Specificity	Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
Predicted Reactivity	Rat, Mouse

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BOS-A00219-datasheet.pdf

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