Antibodies

ATP5B Antibody

Hersteller ProSci
Kategorie
Typ Antibody Polyclonal
Specific against Human, Mouse, Rat
Format Liquid
Applikationen WB, ELISA
Menge 100 ul
Host Rabbit
ArtNr 30-375
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP5B.
Tested Applications
ATP5B antibody can be used for detection of ATP5B by ELISA at 1:312500. ATP5B antibody can be used for detection of ATP5B by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Positive Control 1
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight
52 kDa
Purification
Antibody is purified by protein A chromatography method.
Conjugate
Unconjugated
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
batch dependent
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ATP5B antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
NCBI Official Symbol
ATP5B
Accession #
NP_001677
Protein GI #
32189394
NCBI Gene ID
506
User Note
Optimal dilutions for each application to be determined by the researcher.
NCBI Official Full Name
ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
NCBI Organism
Homo sapiens
Swissprot
P06576
Background
ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5B is the beta subunit of the catalytic core.This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background References 1
Vantourout, P., (2008) Mol. Immunol. 45 (2), 485-492.

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Menge: 100 ul
Lieferbar: In stock
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