Antibodies

TMIGD1 Antibody

Hersteller ProSci
Kategorie
Typ Antibody Polyclonal
Specific against Human, Mouse, Rat
Isotype IgG
Format Liquid
Applikationen WB, IF, ELISA, IHC-P
Menge 0.02 mg
Host Rabbit
ArtNr 7615-0.02
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (72%)
Immunogen
TMIGD1 antibody was raised against an 18 amino acid peptide near the center of human TMIGD1.

The immunogen is located within amino acids 150 - 200 of TMIGD1.
Tested Applications
TMIGD1 antibody can be used for detection of TMIGD1 by Western blot at 1 - 2 μ g/ml. Antibody can also be used for Immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity
TMIGD1 antibody is human, mouse and rat reactive. At least two isoforms of TMIGD1 are known to exist; this antibody will detect both isoforms.
Positive Control 1
Cat. No. 1464 - Rat Liver Tissue Lysate
Predicted Molecular Weight
Predicted: 29 kDa

Observed: 24 kDa
Purification
TMIGD1 antibody is affinity chromatography purified via peptide column.
Conjugate
Unconjugated
Buffer
TMIGD1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL
Storage Conditions
TMIGD1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
NCBI Official Symbol
TMIGD1
Accession #
NP_996663
Protein GI #
45592949
NCBI Gene ID
388364
User Note
Optimal dilutions for each application to be determined by the researcher.
NCBI Official Full Name
transmembrane and immunoglobulin domain containing 1
NCBI Organism
homo sapiens
Swissprot
Q6UXZ0
Background
TMIGD1 (transmembrane and immunoglobulin domain containing 1) is a 262 amino acid single-pass membrane protein that contains two Ig-like C2-type (immunoglobulin-like) domains (1, 2). It is a nonsecreted, glycosylated protein located in the cytoplasm and cell membrane (1). TMIGD1 might be a possible cell-differentiation marker in the intestine, whose expression is lost during cellular transformation and promote progression of tumorigenesis in the intestinal epithelium (2-4). TMIGD1 has also been postulated to play a role in cell adhesion (2).
Background References 1
Clark HF, Gurney AL, Abaya E, et al. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome Res. 2003; 13:2265-70.
Background References 2
Fujimoto K, Beauchamp RD, Whitehead RH. Identification and isolation of candidate human colonic clonogenic cells based on cell surface integrin expression. Gastroenterology 2002; 123:1941–8.
Background References 3
Yan J, Jiang J, Lim CA, et al. Blimp-1 regulates cell growth through repression of p53 transcription. Proc. Natl. Acad. Sci. USA 2007; 104:1841-6.
Background References 4
Cattaneo E, Laczko E, Buffoli F, et al. Preinvasive colorectal lesion transcriptomes correlate with endoscopic morphology (polypoid vs. nonpolypoid). EMBO Mol Med. 2011; 3:334-47.

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Menge: 0.02 mg
Lieferbar: In stock
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