Antibodies

KEAP1 Antibody

Hersteller ProSci
Kategorie
Typ Antibody Polyclonal
Specific against Human
Format Liquid
Applikationen WB, IHC, ELISA
Menge 100 ul
Host Rabbit
ArtNr 28-989
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KEAP1.
Tested Applications
KEAP1 antibody can be used for detection of KEAP1 by ELISA at 1:12500. KEAP1 antibody can be used for detection of KEAP1 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Positive Control 1
Tranfected 293T Cell Lysate
Predicted Molecular Weight
70 kDa, 70 kDa
Purification
Antibody is purified by peptide affinity chromatography method.
Conjugate
Unconjugated
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
batch dependent
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store KEAP1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
NCBI Official Symbol
KEAP1
Accession #
NP_987096
Protein GI #
45269145
NCBI Gene ID
9817
User Note
Optimal dilutions for each application to be determined by the researcher.
NCBI Official Full Name
kelch-like ECH-associated protein 1
NCBI Organism
Homo sapiens
Swissprot
Q14145
Background
KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Background References 1
Padmanabhan, B., (2006) Mol. Cell 21 (5), 689-700.

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Menge: 100 ul
Lieferbar: In stock
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