Antibodies

HIV-1 p24 Antibody [8G9] (biotin)

Hersteller ProSci
Kategorie
Typ Antibody Monoclonal
Specific against other
Clon 8G9
Isotype IgG1
Format Liquid
Applikationen WB, ELISA
Menge 0.02 mg
Host Mouse
ArtNr PM-6335-biotin-0.02
eClass 6.1 32160702
eClass 9.0 42030590
Lieferbar
Immunogen
Mouse monoclonal HIV-1 p24 antibody was raised against a recombinant full-length HIV-1 p24 protein.
Tested Applications
PM-6335-biotin can be used for detection of p24 by Western blot or ELISA at 0.2 – 0.5μ g/mL.
Specificity
By Western blot, anti-HIV-1 p24 antibody detects a ca.24 kDa, a ca.41 kDa, and a ca.55 kDa protein, corresponding to HIV-1 p24 and to its precursors p41 and p55, respectively, in HIV-1 samples.
Positive Control 1
Cat. No. 95-153 - Recombinant HIV-1 p24
Predicted Molecular Weight
Predicted: 24, 41, 55 kDa
Purification
HIV-1 p24 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Conjugate
Biotin
Buffer
HIV-1 p24 Monoclonal Antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration
1 mg/mL
Storage Conditions
HIV-1 p24 Monoclonal Antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
NCBI Official Symbol
GAG
Accession #
AAB50258
Protein GI #
327745
NCBI Gene ID
155030
User Note
Optimal dilutions for each application to be determined by the researcher.
NCBI Official Full Name
gag Pr55(Gag)
NCBI Organism
hiv-1
Swissprot
P04591
Background
HIV-1 p24 Monoclonal Antibody: The human immunodeficiency virus type 1 (HIV-1) particle consists of an envelope, a core and the region between the two termed matrix (1). The HIV-1 Gag protein is a late structural protein that contains four proteins: matrix (p17), capsid (p24), nucleocapsid (p7) and the p6 protein (2). The p24 constitutes the major core component of the virus and shows high degree of sequence conservation among HIV isolates. The Gag p24 has been used as an integral part of multicomponent HIV-1 vaccines (3).
Background References 1
Goto T, Nakai M, and Ikuta K. The life-cycle of human immunodeficiency virus type 1. Micron 1998; 29:123-38.
Background References 2
Freed EO. HIV-1 gag proteins: diverse functions in the virus life cycle. Virology 1998; 251:1-15.
Background References 3
Flynn BJ, Kastenmuller K, Wille-Reece U, et al. Immunization with HIV Gag targeted to dendritic cells followed by recombinant New York vaccinia virus induces robust T-cell immunity in nonhuman primates. Proc. Natl. Acad. Sci. USA 2011; 108:7131–6.

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Menge: 0.02 mg
Lieferbar: In stock
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