Human/Mouse/Rat JNK (T183/Y185) Cell-Based ELISA

Hersteller Raybiotech
Kategorie
Typ Elisa-Kit
Specific against Human, Mouse, Rat
Menge 1 x 96-Well Microplate Kit
ArtNr CBEL-JNK-1
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Quantity options
Short description
RayBio Cell-Based Human/Mouse/Rat JNK (Thr183/Tyr185) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
Target Name and Synonyms
JNK
Solid Support
96-well Microplate
Design Principle
Cell-based
Result Output
Semi-Quantitative
Method of Detection
Colorimetric
Compatible Sample Types
Adherent Cell Culture
Specificity
The antibody pair provided in this kit recognizes human, mouse and rat JNK (pThr183/pTyr185).
Product Features
  • Site and signal pathway-specific
  • In vitro detection of adherent cell culture
  • No sample lysis needed
  • Compatible with a standard ELISA plate reader
  • Faster results than with ELISA
  • Adaptable for high-throughput screening and drug discovery
Kit Components
  • Cell Culture Microplate
  • Wash Buffer A
  • Wash Buffer B
  • Fixing Solution
  • HRP-Conjugated Secondary Antibody
  • Blocking Buffer
  • TMB One-Step Substrate
  • Stop Solution
  • Quenching Buffer
Other Materials Required
  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Protocol Summary
  1. Seed 10, 000-30, 000 cells into each well and incubate overnight.
  2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
  3. Add 100 ul of Fixing Solution into each well and incubate for 20 min at RT with shaking.
  4. Add 200 ul of prepared 1X Quenching Buffer and incubate 20 min at RT.
  5. Add 200 ul of Blocking Solution and incubate for 1 h at 37 C.
  6. Add 50 ul of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
  7. Add 50 ul of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Storage
-20C
Menge: 1 x 96-Well Microplate Kit
Lieferbar: In stock
Listenpreis: 421,00 €
Preis: 421,00 €
lieferbar

Vergleichen

Angebot anfordern

Fragen zum Produkt?
 
Schließen