Recombinant Human BMP-7

Hersteller Raybiotech
Kategorie
Typ Proteins
Specific against Human
Menge 10 ug
ArtNr 229-20181
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Short Description
Purified recombinant BMP-7 expressed in CHO cells.
Description
229-20181 is a 28.8 kDa recombinant protein corresponding to amino acid residues 316 to 431 of the full-length human bone morphogenetic protein 7 (BMP-7) precursor.

BMP-7, also known as Osteogenic protein 1 (OP-1), is a disulphide-linked homodimeric cysteine knot protein and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.
Protein Name
BMP-7
Purity
>98% by SDS PAGE/HPLC analysis
Formulation
Lyophilized from 0.1% TFA
Concentration
Total protein concentration ca.0.1mg/ml after reconstitution.
Preservative
None present
Endotoxin Level
<1EU/ug
Protein Type
Recombinant Protein
Recommended Applications
ELISA (0.2-0.4ng/well), Functional Assays, Western Blotting (1.5-3.0ng/lane)
Biological activity
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.02-0.04 ug/ml.
Reconstitution
Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.
Storage/stability
Prior to reconstitution store at +4° C.
After reconstitution store at -20° C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.
Menge: 10 ug
Lieferbar: Out of stock
Listenpreis: 559,08 €
Preis: 559,08 €
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