Recombinant Mouse NOGGIN

Hersteller Raybiotech
Kategorie
Typ Proteins
Specific against Mouse
Menge 20 ug
ArtNr 229-20309
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Short Description
Purified recombinant Noggin expressed in E.coli
Introduction
Noggin is a secreted polypeptide, originally identified as an antagonist of BMP-4, which binds to and inactivates signal transduction by members of the transforming growth factor-beta (TGF-beta) superfamily, and plays an essential role in cartilage morphogenesis, joint formation and neural tube fusion, as determined by mouse knockout studies. Noggin is implicated in both early and later developmental stages, and can be used as a tool to differentiate human embryonic stem cells into neural precursor cells.
Protein Name
NOGGIN
Molecular Weight (kDa)
46.4kD (206 amino acid residue homodimer)
Purity
>95% by SDS PAGE and HPLC analysis
Formulation
None present
Concentration
ca.0.1mg/ml after reconstitution
Preservative
None present
Endotoxin Level
<1EU/ug
Protein Type
Recombinant Protein
Recommended Applications
Functional Assays
Biological activity
Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is 1.0-2.0 ng/ml of Noggin.
Reconstitution
Reconstitute with 0.2ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution. Further dilutions can be made in water containing 0.1% BSA.
Storage/stability
Prior to reconstitution store at +4° C.
After reconstitution store at -20° C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.
Menge: 20 ug
Lieferbar: Out of stock
Listenpreis: 559,08 €
Preis: 559,08 €
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