|
Hersteller |
Raybiotech
|
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
Rat |
|
Menge |
100 ug |
|
ArtNr |
229-20329 |
|
eClass 6.1 |
34160400 |
|
eClass 9.0 |
42020190 |
|
Lieferbar |
 |
| Short Description |
Purified recombinant EGF expressed in E.coli |
| Introduction |
EGF (Epidermal Growth Factor) is a type I transmembrane protein expressed by a variety of cells, which acts as a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, and is a key factor in cell survival.
The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the EGF receptor (EGFR), which results in the triggering of several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways. |
| Protein Name |
EGF |
| Molecular Weight (kDa) |
6.2kD (54 amino acid residues) |
| Purity |
>98% by SDS PAGE and HPLC analysis |
| Concentration |
ca.0.1mg/ml |
| Preservative |
None present |
| Endotoxin Level |
<1EU/ug |
| Protein Type |
Recombinant Protein |
| Recommended Applications |
Functional Assays |
| Biological activity |
The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg. |
| Reconstitution |
Reconstitute with 1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution. |
| Storage/stability |
Prior to reconstitution store at +4° C.
After reconstitution store at -20° C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution. |
