Purified Rat FIBRONECTIN

Hersteller Raybiotech
Kategorie
Typ Proteins
Specific against Rat
Menge 1 mg
ArtNr 229-20332
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Introduction
Fibronectin is a 450kDa glycoprotein involved in cell adhesion and migration processes including embryogenesis, metastasis, wound healing, blood coagulation and host immunity.

Fibronectin may be used as a coating on tissue culture surfaces to promote attachment, spreading and proliferation of various cell types.
Protein Name
FIBRONECTIN
Purity
>/=95% by SDS PAGE
Concentration
Protein concentration ca.0.5 mg/ml
Preservative
None present
Buffers
Phosphate buffered saline
Protein Type
Purified Protein
Recommended Applications
ELISA, Tissue Culture (1-10 ug/ml)
Instructions For Use
Preparation of Fibronectin:


Generally fibronectin should be stored frozen or refrigerated at 1-5 mg/ml. If frozen, thaw at 37° C. Fibronectin should never be vortexed or treated roughly as it precipitates out of solution easily (visible as a granulated precipitate or as a slimy strand or clumps). Any observed insoluble aggregates are very difficult to resuspend. If precipitation is observed, the solution should initially be warmed to 37° C with gentle shaking, followed by aseptic filtration via a 5 µ m (or smaller) cut off syringe filter.



It is also recommended that fibronectin should not be stored long term in buffer solutions containing Mg2+ or Ca2+ as these can contribute to fibronectin precipitation over time.



General Coating Procedure (for 96-well plates):


1. Plates: Choice of plates can affect the amount of protein successfully coated. RayBiotech recommends the use of commercially available high protein binding plates.



2. Add between 1-10 µ g/ml of fibronectin in PBS buffer for an overnight (or longer) incubation at 2-8° C (100 µ l/well). The optimum amount of protein to saturate the plate surface should be determined by the user. For ELISA applications some users have coated with carbonate buffer pH 9.0 but this is generally not necessary and can damage the cell binding activity of the fibronectin.



3. Regardless of assay type (cell binding, ELISA etc.), it is necessary to block the remaining protein adherence sites on the plate. Therefore, in a separate step add a blocking protein such as 2-5% BSA in PBS for at least 1 hr at room temperature or overnight at 2-8° C (200 µ l/well). Caution: The BSA solution should be 0.2 µ m filtered prior to use to remove excess non-specific binding effects in the assay caused by insoluble BSA clumps. The use of RIA-grade (radio-immunoassay grade, suitable for immunoassays) BSA is recom
Storage/stability
Store at +4° C or at -20° C for long term storage.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. This protein may be stored for18 months from date of dispatch.
Menge: 1 mg
Lieferbar: Out of stock
Listenpreis: 628,00 €
Preis: 628,00 €
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