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Human DPEP2 HEK293 Overexpression Lysate

Human DPEP2 HEK293 Overexpression Lysate

Hersteller	Sino Biological
Kategorie	Cell Lines & Cell Culture Cell Lysates
Typ	Cell Lysate
Specific against	Human
Format	Human Cells
Menge	300ug
Host	Human
ArtNr	13806-H02HL-300
eClass 6.1	32190290
eClass 9.0	32190290
Lieferbar
Molecule	DPEP2
Product Description	Human Cells transfected lysate in which Human MBD2 / DPEP2 has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer.
Expressed Host	Human Cells
Preparation Method	Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer	Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality Control Testing	12.5% SDS-PAGE Stained with Coomassie Blue
Recommend Usage	1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200uL pure water and boiled for 2-5 min. 3. Store it at -80C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. Notes: The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer	In modified RIPA Lysis Buffer
Storage Instruction	Store at -80C. Aliquot to avoid repeated freezing and thawing
Stability	Samples are stable for up to twelve months from date of receipt at -80C
Application notes	WB: Use at an assay dependent dilution. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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13806-H02HL-300-datasheet.pdf

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Menge: 300ug

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Lieferung vsl. bis 09.05.2024

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