Human TFPI2 Protein (His Tag)

Hersteller Sino Biological
Kategorie
Typ Proteins
Specific against Human
Format Human Cell
Menge 20 ug
Host Human
ArtNr 10545-H08H-20
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Molecule
TFPI2
Synonym
PP5, REF1, TFPI-2
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
A DNA sequence encoding the human TFPI2 (NP_006519.1) precursor (Met 1-Lys 213) was expressed with a C-terminal polyhistidine tag.
Expressed Host
Human Cells
Purity
> 95 % as determined by SDS-PAGE
Bio-Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114) . The IC50 value is < 2 nM, as measured in 100uL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 uM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl , 0.05% Brij-35, pH 7.5.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Asp 23
Molecule Mass
The secreted recombinant humanTFPI2 comprises 202 amino acids and predicts a molecular mass of 23.2 kDa. It migrates as an approximately 32-34 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Accession
NP_006519.1
Amino acid sequence
Met1-Lys213
TAG
C-His
Menge: 20 ug
Lieferbar: In stock
Listenpreis: 318,90 €
Preis: 318,90 €
lieferbar

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