Human TFPI Protein (His Tag)

Hersteller Sino Biological
Kategorie
Typ Proteins
Specific against Human
Format Human Cell
Menge 10 ug
Host Human
ArtNr 10564-H08H-10
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Molecule
TFPI
Synonym
EPI, LACI, TFI, TFPI1
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
A DNA sequence encoding the human TFPI (NP_006278.1) (Met 1-Lys 282) was expressed with a C-terminal polyhistidine tag.
Expressed Host
Human Cells
Purity
> 85 % as determined by SDS-PAGE
Bio-Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114) . The IC50 value is < 0.4 nM, as measured in 100uL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 uM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5 .
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Asp 29
Molecule Mass
The secreted recombinant human TFPI comprises 265 amino acids with a predicted molecular mass of 30.6 kDa. As a result of glycosylation, the apparent molecular mass of rhTFPI is approximately 42-45 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Accession
NP_006278.1
Amino acid sequence
Met1-Lys282
TAG
C-His
Menge: 10 ug
Lieferbar: In stock
Listenpreis: 192,96 €
Preis: 192,96 €
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