ELISA Kit for Cyanocobalamin (CNCbl)

Kein Verkauf an Patienten, Ärzte, Apotheken oder Privatpersonen! Mehr erfahren

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against General
Applikationen ELISA
Menge 96T
ArtNr CEA924Ge
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Cyanocobalamin
Alternative Names
VB12, Vitamin B12, Cobalamin, alpha-5, 6-Dimethylbenzimidazolyl Cobamidcyanide
Detection range
123.5-10, 000pg/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<50.5pg/mL
Sample type
serum, plasma, tissue homogenates and other biological fluids
Assay length
2h
Method
Competitive Inhibition
Specificity
This assay has high sensitivity and excellent specificity for detection of Cyanocobalamin (CNCbl).
No significant cross-reactivity or interference between Cyanocobalamin (CNCbl) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyanocobalamin (CNCbl) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyanocobalamin (CNCbl) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add50µ Lstandardorsampletoeachwell.
        Andthenadd50µ LpreparedDetectionReagentAimmediately.
        Shakeandmix.Incubate1hourat37° C;
3.Aspirateandwash3times;
4.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
5.Aspirateandwash5times;
6.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
7.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cyanocobalamin (CNCbl) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cyanocobalamin (CNCbl) and unlabeled Cyanocobalamin (CNCbl) (Standards or samples) with the pre-coated antibody specific to Cyanocobalamin (CNCbl). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cyanocobalamin (CNCbl) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cyanocobalamin (CNCbl) in the sample.
Research Area
Metabolic pathway, Nutrition metabolism,
References
Vitamin Status and the Development of Postoperative Cognitive Decline in Elderly Surgical Oncologic Patients.
Selected Risk Nutritional Factors for Chemotherapy-Induced Polyneuropathy.
Lettore di micropiastre multimodale

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt und Sicherheitsdatenblatt) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Menge: 96T
Lieferbar: In stock
lieferbar

Vergleichen

Auf den Wunschzettel

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen