ELISA Kit for Tetrahydrofolic Acid (THFA)

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Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against General
Applikationen ELISA
Menge 96T
ArtNr CEG411Ge
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Tetrahydrofolic Acid
Alternative Names
H4FA, THF, Tetrahydrofolate
Detection range
1.23-100ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<0.57ng/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
2h
Method
Competitive Inhibition
Specificity
This assay has high sensitivity and excellent specificity for detection of Tetrahydrofolic Acid (THFA).
No significant cross-reactivity or interference between Tetrahydrofolic Acid (THFA) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tetrahydrofolic Acid (THFA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tetrahydrofolic Acid (THFA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add50µ Lstandardorsampletoeachwell.
        Andthenadd50µ LpreparedDetectionReagentAimmediately.
        Shakeandmix.Incubate1hourat37° C;
3.Aspirateandwash3times;
4.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
5.Aspirateandwash5times;
6.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
7.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tetrahydrofolic Acid (THFA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tetrahydrofolic Acid (THFA) and unlabeled Tetrahydrofolic Acid (THFA) (Standards or samples) with the pre-coated antibody specific to Tetrahydrofolic Acid (THFA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tetrahydrofolic Acid (THFA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tetrahydrofolic Acid (THFA) in the sample.
References
Assessment of Folic Acid Supplementation in Pregnant Women by Estimation of Serum Levels of Tetrahydrofolic Acid, Dihydrofolate Reductase, and Homocysteine

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Menge: 96T
Lieferbar: In stock
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