Vergleich

High Sensitive ELISA Kit for Interleukin 8 (IL8)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96T
ArtNr HEA080Hu
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Quantity options
Detection range
3.9-250pg/mL
Organism species
Homo sapiens (Human)
Sensitivity
The minimum detectable dose of this kit is typically less than 1.7pg/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity

This assay has high sensitivity and excellent specificity for detection of High Sensitive Interleukin 8 (IL8).
No significant cross-reactivity or interference between High Sensitive Interleukin 8 (IL8) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Interleukin 8 (IL8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 8 (IL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 8 (IL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interleukin 8 (IL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Interleukin 8 (IL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Cytokine; Infection immunity;

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Menge: 96T
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 04.11.2022 

Vergleichen

Auf den Wunschzettel

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen