ELISA Kit for Deiodinase, Iodothyronine, Type II (DIO2)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Mouse
Applikationen ELISA
Menge 96T
ArtNr SEC903Mu
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Detection range
0.781-50ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Organism species
Mus musculus (Mouse)
Sensitivity
The minimum detectable dose of this kit is typically less than 0.28ng/mL
Sample type
Tissue homogenates and other biological fluids.
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Deiodinase, Iodothyronine, Type II (DIO2).
No significant cross-reactivity or interference between Deiodinase, Iodothyronine, Type II (DIO2) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Deiodinase, Iodothyronine, Type II (DIO2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Deiodinase, Iodothyronine, Type II (DIO2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deiodinase, Iodothyronine, Type II (DIO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deiodinase, Iodothyronine, Type II (DIO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deiodinase, Iodothyronine, Type II (DIO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deiodinase, Iodothyronine, Type II (DIO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Alternative Names
5DII, D2, SelY, TXDI2, Thyroxine Deiodinase, Type II, Type-II 5'-deiodinase
Item Name
Deiodinase, Iodothyronine, Type II
Research Area
Enzyme & Kinase,
Menge: 96T
Lieferbar: In stock
Listenpreis: 553,85 €
Preis: 553,85 €
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Email: sales@hoelzel.de

Arbeitsanleitung

SEC903Mu.pdf