ELISA Kit for Fibroblast Growth Factor 21 (FGF21)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Rat
Menge 96T
ArtNr SEC918Ra
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Detection range
7.813-500pg/mL The standard curve concentrations used for the ELISA’s were 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, 7.813pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 2.9pg/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length
4.5hours
Specificity
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 21 (FGF21)
No significant cross-reactivity or interference between Fibroblast Growth Factor 21 (FGF21) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1. Prepare all reagents, samples and standards,
2. Add 100µ, L standard or sample to each well. Incubate 2 hours at 37oC,
3. Aspirate and add 100µ, L prepared Detection Reagent A. Incubate 1 hour at 37oC,
4. Aspirate and wash 3 times,
5. Add 100µ, L prepared Detection Reagent B. Incubate 30 minutes at 37oC,
6. Aspirate and wash 5 times,
7. Add 90µ, L Substrate Solution. Incubate 15-25 minutes at 37oC,
8. Add 50µ, L Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 21 (FGF21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 21 (FGF21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 21 (FGF21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±, 10nm. The concentration of Fibroblast Growth Factor 21 (FGF21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Arbeitsanleitung

SEC918Ra.pdf

Menge: 96T
Lieferbar: Out of stock
Listenpreis: 584,62 €
Preis: 584,62 €
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