Recombinant Peptidylprolyl Isomerase F (PPIF)

Hersteller Cloud-Clone
Kategorie
Typ Proteins Recombinant
Specific against Mouse
Applikationen WB, IP, ELISA
Menge 10ug
Host E.coli
ArtNr RPB549Mu01-10ug
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Purity
≥ 95%
Predicted Molecular Mass
19.9kDa
Concentration
9
Endotoxin Level
< 1.0EU per 1µ g (determined by the LAL method)
Formulation
Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Effective Size Range
10kDa to 70kDa.
Protein bands
10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands
The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use
No need to heat, dilute or add reducing agents before use.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NPLVYLD VGADGQPLGR VVLELKADVV PKTAENFRAL CTGEKGFGYK GSTFHRVIPA FMCQAGDFTN HNGTGGRSIY GSRFPDENFT LKHVGPGVLS MANAGPNTNG SQFFICTIKT DWLDGKHVVF GHVKEGMDVV KKIESFGSKS GK
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8° C for one month. Aliquot and store at -80° C for 12 months.
Stability Test
The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37° for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Usage
Reconstitute in sterile PBS, ph7.2-pH7.4.
Menge: 10ug
Lieferbar: In stock
Listenpreis: 167,79 €
Preis: 167,79 €
lieferbar

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