Antibodies

CBFB

Manufacturer Abclonal
Category Products / Antibody
Type Antibody Polyclonal
Specific against Human, Mouse, Rat
Isotype IgG
Applications WB, IF, IHC
Amount 100ug
Host Rabbit
ArtNr A5305-100ug
Eclass 6.1 32160702
Eclass 9.0 32160702
Available
Background
The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
CBFB
Gene Template
Human
GeneID
865
Route
Recombinant Protein
SWISS
Q13951
Protein MW (aa)
22kDa
Synonyms
CBFB, CBF beta, PEBP2B, PEA2 beta, PEBP2 beta,
Purity
Affinity purification
Storage Buffer
Store at -20C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Recommended Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Illustration
Western blot analysis of extracts of various cell lines, using CBFB antibody. Immunohistochemistry of paraffin-embedded human stomach cancer using CBFB antibody at dilution of 1:200 (x400 lens). Immunohistochemistry of paraffin-embedded Mouse brain using CBFB antibody at dilution of 1:100 (x400 lens). Immunofluorescence analysis of U2OS cell using CBFB antibody. Blue: DAPI for nuclear staining.
Amount: 100ug
Available: In stock
Listprice: €263.00
Price: €263.00
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Instruction sheet

A5305-100ug.pdf