Trypsin-1 Human Recombinant

Lyophilized powder at room temperature.

Trypsin-1 Human Recombinant produced in CHO cells is a 24.1kDa single polypeptide chain consisting of 224 amino acids.
Amino Acid Sequence:IVGGYNCEEN SVPYQVSLNS GYHFCGGSLI NEQWVVSAGH CYKSRIQVRL GEHNIEVLEG NEQFINAAKI IRHPQYDRKT LNNDIMLIKL SSRAVINARV STISLPTAPP ATGTKCLISG WGNTASSGAD YPDELQCLDA PVLSQAKCEA SYPGKITSNM FCVGFLEGGK DSCQGDSGGP VVCNGQLQGV VSWGDGCAQK NKPGVYTKVY NYVKWIKNTI AANS.
Source: Chinese Hamster Ovary cells.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: The Trypsin-1 filtered 0.22um solution contains 20mM TRIS-HCl buffer pH 7.2. Greater than 95% as determined by SDS-PAGE.
Biological Activity: 1 unit hydrolyzes 1 umole of Na-Benzoyl-D, L-arginine 4-nitroanilide (BANI) per minute at 37C, pH 8.0, in the presence of 10mM calcium.
The specific activity is > 1, 000 Units per mg protein as measured under the described conditions.