Comparison

Anti-MIF Antibody Picoband® European Partner

Item no. BOS-RP1020-Cy3
Manufacturer Boster
Amount 100 ug
Quantity options 100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category
Type Antibody Polyclonal
Format Lyophilized
Applications WB, ELISA
Specific against Human (Homo sapiens)
Host Rabbit
Isotype IgG
Conjugate/Tag Cy3
Sensitivity >5000 cells
Sequence Belongs to the MIF family.
Citations 1. Bernhagen, J.; Krohn, R.; Lue, H.; Gregory, J. L.; Zernecke, A.; Koenen, R. R.; Dewor, M.; Georgiev, I.; Schober, A.; Leng, L.; Kooistra, T.; Fingerle-Rowson, G.; Ghezzi, P.; Kleemann, R.; McColl, S. R.; Bucala, R.; Hickey, M. J.; Weber, C. : MIF is a noncognate ligand of CXC chemokine receptors in inflammatory and atherogenic cell recruitment. Nature Med. 13: 587-596, 2007.
2. Bozza, M.; Kolakowski, L. F., Jr.; Jenkins, N. A.; Gilbert, D. J.; Copeland, N. G.; David, J. R.; Gerard, C. : Structural characterization and chromosomal location of the mouse macrophage migration inhibitory factor gene and pseudogenes. Genomics 27: 412-419, 1995
3. Budarf, M.; McDonald, T.; Sellinger, B.; Kozak, C.; Graham, C.; Wistow, G. : Localization of the human gene for macrophage migration inhibitory factor (MIF) to chromosome 22q11.2. Genomics 39: 235-236, 1997.
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Macrophage migration inhibitory factor,MIF,5.3.2.1,Glycosylation-inhibiting factor,GIF,L-dopachrome isomerase,L-dopachrome tautomerase,5.3.3.12,Phenylpyruvate tautomerase,MIF,GLIF,MMIF
Available
Manufacturer - Category
Primary Antibodies
Storage Conditions
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight
12 kDa
Calculated Molecular weight
12476 MW
Clonality
Polyclonal
Application Details
Western blot, 0.1-0.5μg/ml, Human, -
ELISA, 0.1-0.5μg/ml
Gene Name
MIF
Gene Full Name
Macrophage migration inhibitory factor
Manufacturer - Specificity
No cross reactivity with other proteins.
Background
Macrophage migration inhibitory factor, MIF, is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1kb. Localization of the human gene for macrophage migration inhibitory factor (MIF) to chromosome 22q11.2. MIF plays a critical role in inflammatory diseases and atherogenesis.
Immunogen
E. coli-derived human MIF recombinant protein (Position: M1-A115).
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Purification
Immunogen affinity purified.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category
Immunology, Innate Immunity, Macrophage/Inflammation
Protein Name
Macrophage migration inhibitory factor
Protein Function
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti- inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Subcellular Localization
Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non- classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
Description
Boster Bio Anti-MIF Antibody catalog # RP1020. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity
Restricted to a subset of activated T-cells.
Sequence Similarities
Belongs to the MIF family.
Manufacturer - Cross Reactivity
No cross-reactivity with other proteins

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
available

Delivery expected until 12/4/2025 

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