Histone H4, trimethyl (Lys20) (Biotin)

Antibodies / Antibodies-Histones

-20°C

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

Synthetic peptide containing the sequence …HR[me3K]VL, in which me3K corresponds to trimethyl-lysine 20 of human histone H4. Coupled to BSA.

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H4 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Applications:
Suitable for use in Western Blot, Dot Blot and Chromatin Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot:2ug/ml detected methylated histone H4 in acid extracted proteins from 10ug HeLa cells, but not demethylated recombinant Histone H4.
Chromatin Immunoprecipitation: Has been reported to immunoprecipitate chromatin containing trimethylhistone H4 (Lys20).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.