WDR77 (Methylosome Protein 50, MEP-50, WD Repeat-containing Protein 77, Androgen Receptor Cofactor p44, p44/Mep50, MEP50, WD Repeat Domain 77) (Azide Free)

Antibodies / Antibodies-WD Repeat Proteins

-20°C

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).

Recombinant protein corresponding to a portion of amino acids within aa1-343 of human WDR77.

WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM]

Applications:
Suitable for use in ELISA, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1, 000
Immunohistochemistry (paraffin): 1.5ug/ml
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.