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Anti-Rat IL-4 - 25 µg

Anti-Rat IL-4 - 25 µg

Item no.	LEIN-I-225-25ug
Manufacturer	Leinco Technologies
Amount	25 ug
Quantity options	100 ug 25 ug
Category	Antibodies Western Blot Primary Antibodies
Type	Antibody Primary
Applications	WB
Specific against	Rat (Rattus norvegicus)
Host	Caprine
Citations	1. Pike, JW. et al. (2001) PNAS 98: 2443 2. Finkelman, FD. et al. (2003) J Immunol. 170: 3835 3. Kaplan, MH. et al. (2008) J Immunol. 180: 3551
NCBI	287287
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	N, WB
Manufacturer - Category	Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets	IL-4
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	IL-4 is a 15-19 kD pleiotropic immune cytokine secreted by activated TH2 cells. It has many biological roles, including the regulation in humoral and adaptive immunity, the inhibition of bone resorption, the stimulation of activated B-cell and T-cell proliferation, and initiation of allergic responses. Moreover, IL-4 induces a positive feedback loop by stimulating differentiation of naive helper T cells, or Th0 cells, to Th2 cells which in turn secrete more IL-4. Increased IL-4 production and overproduction of IL-4R are found in many cancers. IL-4 has a significant effect on tumor progression by increasing apoptosis resistance via tumor growth enhancement. IL-4 may also play a part in the immune dysregulation that occurs in most HIV-1 infected patients.
Background	IL-4 is a 15-19 kD pleiotropic immune cytokine secreted by activated TH2 cells. It has many biological roles, including the regulation in humoral and adaptive immunity, the inhibition of bone resorption, the stimulation of activated B-cell and T-cell proliferation, and initiation of allergic responses. Moreover, IL-4 induces a positive feedback loop by stimulating differentiation of naive helper T cells, or Th0 cells, to Th2 cells which in turn secrete more IL-4. Increased IL-4 production and overproduction of IL-4R are found in many cancers. IL-4 has a significant effect on tumor progression by increasing apoptosis resistance via tumor growth enhancement. IL-4 may also play a part in the immune dysregulation that occurs in most HIV-1 infected patients.
Other Applications Reported In Literature	Neutralization:Measured by its ability to neutralize IL‑4-induced proliferation in the rat splenocytes. The Neutralization Dose (ND₅₀) is typically 0.05-015 µg/mL in the presence of 4 ng/mL Recombinant Rat IL‑4 and 10 µg/mL PHA.
PubMed	IL-4
Manufacturer - Research Area	Other Molecules
Manufacturer - Specificity	Rabbit Anti-Rat Interleukin-4 (IL-4) recognizes Rat IL-4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2830577
Formulation	This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Rat IL-4 (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, Interleukin-4, BCGF, BCDF, B Cell Stimulating Factor, BSF-1, IL-4, Rat IL-4, Anti-Rat IL-4, B cell growth factor 1, BCDF, B-cell stimulatory factor 1, BCGF1, BCGF-1, binetrakin, BSF1, BSF-1, IL4, IL-4, IL-4B_cell stimulatory factor 1, interleukin 4, interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, pitrakinra

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-225-25ug-safety-datasheet.pdf