Anti-Human Tie-2 - 25 µg

B, IHC FFPE, WB

Tie-2

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Tyrosine kinase with immunoglobulin-like and EGF-like domains 2 (Tie-2) are novel endothelial cell tyrosine kinase receptors that are essential for vascular development and remodeling in the embryo.1 They are expressed almost exclusively in endothelial cells.2 Tie-2 is functional in pericytes and may play an important role in the progression of diabetic retinopathy, by regulating pericyte loss and influencing the activation state and recruitment of pericytes.3 Tie2 has been shown to be up-regulated in psoriasis and in breast cancer tissue and blocking Tie2 action inhibits tumor angiogenesis and tumor growth.

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 3-10 µg/ml for 5-15 µm thick sections. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Blocking: Neutralization of receptor-ligand interaction - Approximately 5-10 μg/mlof this antibody will block 50% of the binding of 40 ng/ml of recombinant human Angiopoietin-2 to immobilized recombinant human Tie-2/Fc chimera (100 μl of a 4 μg/ml solution was coated in each well) in a functional ELISA assay.

Goat Anti-Human Tie-2 recognizes Human Tie-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.