Comparison

 

Use the following products to optimize your experiment:

 

 
Fertiup cryoprotectant
 
Fertiup Preincubation Medium
 
Card Medium
 
Card HyperOva

Benefits of the newly developed system for sperm cryopreservation and IVF:

  •  Superior support for the safe and reliable preservation of your valuable mouse strains
  •  High-performance media for mouse sperm cryopreservation, sperm preincubation and In vitro fertilization (IVF)
  •  Provide high and stable fertilization rate using frozen-thawed mouse sperm
  •  FERTIUP® and CARD MEDIUM® have no reproduction toxicity of any kind

 

 


 

More information about Assisted Reproductive Technologies and all relevant products:

 

 

Assisted Reproductive Technologies

 

CONTENT:

  1. Introduction
  2. Overview
  3. CARD MEDIUM® und FERTIUP®
  4. CARD HyperOva® the superovulation reagent
  5. Peripheral products
  6. Products

Introduction

In recent years, the number of genetically engineered mice being produced has increased dramatically. Moreover, the rapid progress in the development of genome-editing (TALEN and CRISPR/Cas9) techniques for molecular biology reserach has been remarkable, so much so that a genetically engineered mouse strain can be produced easily in a few months. Production has been supported by reproductive engineering techniques such as In vitro fertilisation, embryo and sperm cryopreservation and embryo transfer techniques. Such techniques have become invaluable peripheral technologies and their use is expanding rapidly. 

Fig. 1: Lebenszyklus einer Maus bei Nutzung von FERTIUP® und CARD MEDIUM®

Overview

To support your research we offer several reagents and disposable items (general labware) for In vitro fertilisation, In vitro culture, cryopreservation and In vitro manipulation.

CARD MEDIUM® and FERTIUP®

Sperm cryopreservation is a practical and cost-effective method of archiving mouse strains in the laboratory. FERTIUP® and CARD MEDIUM® are high-performance media for mouse sperm cryopreservation, sperm preincubation and In vitro fertilization (IVF). 


When used in combination, FERTIUP® and CARD MEDIUM® provide a high and stable fertilization rate using frozen-thawed mouse sperm. This will allow you to reproduce mice whenever you need them.
The FERTIUP® sperm cryoprotectant and sperm preincubation medium greatly improve fertilization rates of frozen-thawed sperm in C57BL/6 and 129 Mice strains, from less than 10% (using conventional methods) to more than 30%. 
In addition, CARD MEDIUM® enhances the fertility rate to over 80%. FERTIUP® and CARD MEDIUM® have no reproduction toxicity of any kind. 


The newly developed system for sperm cryopreservation and IVF using FERTIUP® and CARD MEDIUM® provides superior support for the safe and reliable preservation of your valuable mouse strains.

No.StrainsMethodNumber of
oocytet donors
Number of
inseminated oocytes
Average number of
ovulated oocytes/female
No. of
fertilized eggs
Fertilization rate (%)
1 C57BL/6J eCG
Product
10
10
270
1072
27.7±5.4
107.2±22.7
267
963
96.4±3.2
89.8±3.7
2 BALB/c ByJ eCG
Product
10
10
293
903
29.3±8.9
90.3±14.5
213
680
73.4±11.3
76.4±9.0
3 C3H/HeJ eCG
Product
10
10
286
520
28.6±8.4
52.0±18.1
251
446
87.8±22.6
85.8±15.5
4 DBA/2J eCG
Product
10
10
225
688
22.5±7.8
68.8±13.6
194
592
86.2±11.0
86.0±4.5
5 FVB/NJ eCG
Product
10
10
168
256
16.8±3.3
25.6±5.9
159
239
94.6±6.7
93.3±6.6
6 CD1 eCG
Product
10
10
202
337
20.2±8.0
33.7±9.9
146
269
72.3±16.0
79.8±19.6

Fig. 2:

  • 7.5 IU of CARD HyperOva® or PMSG was injected to four weeks old female mice, then 7.5 IU of hCG was administered 48 hours later.
    Thereafter all ova obtained 17 hours after the hCG injection were collected.
  • The In vitro fertilization rate is defined as the number of 2-cell embryos divided by the total number of ova (mean±SD).
  • Data cited from a paper published by Prof. Naomi Nakagata and Toru Takeo (Center for Animal Resources and development, Kumamoto University)
Fig. 3: Fertilization rates using FERTIUP® Preincubation Medium and CARD MEDIUM® for fresh and frozen sperm in various strains.
Fig. 4: Fertilization rates using frozen-thawed sperm with FERTIUP® and with FERTIUP® and CARD MEDIUM®

CARD HyperOva® the superovulation reagent

Reduction in experimental animal use (Contribution to 3Rs):
Using CARD HyperOva® enables us to reduce the number of female mice used for ovum collection by 25 - 30 %.

Optimization of genetically modified mouse creation and preservation:
More ova can be obtained from less female mice using CARD HyperOva® than following the conventional method, which will facilitate In vitro fertilization and embryo transfer.

Application to valuable genetically modified mice:
Using CARD HyperOva® on your valuable genetically modified mice enables you to obtain a lot of ova from small number of females, thus enabling the large-scale preparation of embryos.

Periphere products

Additionally to reagents regarding reproductive engineering techniques we offer several instruments. For example a transportation kit for the cauda epididymides or straws to execute In vitro fertilisation steps.

Products

Save 15% with our sets

NameTypeProductno.Components
FERTIUP Mouse Sperm Cryoprotectant and Preincuabtion Medium (KYD-001-05-EX & KYD-002-05-EX) Kit

KYD-SET-01

KYD-001-05-EX
KYD-002-05-EX
FERTIUP Mouse Sperm Cryoprotectant and Preincuabtion Medium + Card HyperOva (KYD-001-05-EX & KYD-002-05-EX & KYD-010-EX) Kit

KYD-SET-02

KYD-001-05-EX
KYD-002-05-EX
KYD-010-EX
FERTIUP Mouse Sperm Cryoprotectant and Preincuabtion Medium + Card Medium + Card HyperOva (KYD-001-05-EX & KYD-002-05-EX & KYD-003-EX & KYD-010-EX) Kit

KYD-SET-03

KYD-001-05-EX
KYD-002-05-EX
KYD-003-EX
KYD-010-EX
FERTIUP Mouse Sperm Cryoprotectant and Preincuabtion Medium (KYD-001-05-EX & KYD-002-05-EX) Kit

KYD-SET-04

KYD-001-05-EX
KYD-002-05-EX

Media for In vitro Fertilisation

NameTypeProductno.AmountReferences
For mouse        
KSOM In vitro fertilisation

CSR-R-B075
CSR-R-B074

10*5 ML
10*2 ML
 
mHTF In vitro fertilisation

KYD-008-02-EX
KYD-008-02-EX-X5
KYD-008-05-EX
KYD-008-05-EX-X3

2 ML
5*2 ML
5 ML
3*5 ML
Kito et al., 2004
CARD MEDIUM® In vitro fertilisation KYD-003-EX 1 Kit Takeo et al.,  2011
Takeo et al., 2010
FERTIUP® PM Card Medium Set In vitro fertilisation

KYD-004-EX
KYD-005-EX

1 Set
1 Set
 

Media for In vitro culture

NameTypArtikelnr.MengeReferenz
For mouse        
KSOM In vitro culture

CSR-R-B075
CSR-R-B074

10*5 ML
10*2 ML
Lawitts et al., 1993
mWM In vitro culture

CSR-R-B081
CSR-R-B080

10*5 ML
10*2 ML
 
0.25 M Sucrose In vitro culture

CSR-R-Y078
CSR-R-Y077

10*5 ML
10*2 ML
Nakao et al., 1997
For rat
mR1ECM In vitro culture

CSR-R-M174
CSR-R-M191

10*5 ML
10*2 ML
 

Media for preincubation of cryopreserved spermatocytes

NameTypArtikelnr.MengeReferenz
FERTIUP® Preincubation Medium: PM In vitro fertilisation

KYD-002-EX
KYD-002-EX-X5
KYD-002-05-EX
KYD-002-05-EX-X5

1 ML
5*1 ML
0.5 ML
5*0.5ML
 

Media for cryopreservation

NameTypArtikelnr.MengeReferenz
FERTIUP® Cryoprotectant: CPA Cryopreservation

KYD-001-EX
KYD-001-EX-X5
KYD-001-05-EX
KYD-001-05-EX-X5

1 ML
5*1 ML
0.5 ML
5*0.5 ML
 
1M DMSO Cryopreservation CSR-R-T072 10*2 ML Nakao et al., 1997
DAP213 Cryopreservation CSR-R-T073  10*1 ML  

Media for embryo manipulation

NameTypArtikelnr.MengeReferenz
PEPeS Cryopreservation CSR-R-P187 10*1 ML  
P10 Cryopreservation CSR-R-P186 10*2 ML  
M2 In vitro manipulation CSR-R-M084
CSR-R-M083
10*5 ML
10*2 ML
 
PB1 In vitro manipulation CSR-R-P138
CSR-R-P185
10*5 ML
10*2 ML
Nakao et al., 1997

Media for an enhanced superovulation

NameTypArtikelnr.MengeReferenz
CARD HyperOva® In vitro fertilisation KYD-010-06-EX
KYD-010-06-EX-X5
KYD-010-EX
KYD-010-EX-X5
5*1ML

Takeo et al., 2016
Takeo et al., 2015
Zitate:
Noda et al., 2017
Nakagawa et al., 2016
Nakagawa et al., 2017

Disposable Items and instruments for murine reproductive engineering

NameTypArtikelnr.MengeReferenz
Sperm Straws (10 pieces x 10 Units) Disposable items, General labware KYD-S020X10    
Freezing Canister Disposable items, General labware KYD-S018 10 Units  
Straw Connector (5 parts included) Disposable items, General labware KYD-S025 1 Unit  
Triangular Cassette Short (10 units) Disposable items, General labware KYD-S021 10 Units  
Triangular Cassette Long (10 units) Disposable items, General labware KYD-S035 10 Units  
Embryo Manipulation Instrument Set Disposable items, General labware KYD-S036 1 Set  
Glass capillary 20PC Disposable items, General labware KYD-S037 1 Set  
Touch Burner APT-3 Disposable items, General labware PHD-APT3-EX 1 Unit  
CARD Cold Transport Kit Disposable items, General labware KYD-006-EX 1 Set  

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