Comparison

Recombinant Human Ephrin type-B receptor 1 (EPHB1), partial (Active)

Manufacturer Biomatik
Category
Type Proteins Recombinant
Specific against Human
Amount 50ug
Item no. BM-RPC26996-50ug
eClass 6.1 34160400
eClass 9.0 42020190
Available
Gene Name
EPHB1
Alternative Names
Ephrin Type-B Receptor 1, ELK, EPH Tyrosine Kinase 2, EPH-Like Kinase 6, EK6, hEK6, Neuronally-Expressed EPH, Related Tyrosine Kinase, NET, Tyrosine-Protein Kinase Receptor EPH-2, EPHB1, ELK, EPHT2, HEK6
Uniprot
P54762
Source
Mammalian cell
Expression Region
564-984aa
AA Sequence
SRKRAYSKEAVYSDKLQHYSTGRGSPGMKIYIDPF TYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEV YKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSE ASIMGQFDHPNIIRLEGVVTKSRPVMIITEFMENG ALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLAEM NYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDT SDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVW SYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRL PPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTL DKMIRNPASLKTVATITAVPSQPLLDRSIPDFTAF TTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTS EDLLRIGITLAGHQKKILNSIHSMRVQISQSPTAM
Sequence Info
Partial
Tag Info
C-terminal 6xHis-tagged
Theoretical MW
48.8 kDa
Purity
>95% as determined by SDS-PAGE.
Storage Buffer
Lyophilized from a 0.2 um Filtered 20 mM Tris-HCl, 150 mM NaCl, pH 8.0
Endotoxin Level
Less than 1.0 EU/ug as determined by LAL method.
Biological Activity
The ED50 as determined by its ability to binding Human EFNB2 used funtional ELISA is less than 100 ug/ml.
Shipping Condition
Ice packs
Storage
Short term: -20CC; Long term: -80CC. Minimize freeze and thaw cycles.
Expiry Date
1 year
Restriction
For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Relevance
Ephrin Type-B Receptor 1 (EPHB1) is a single-pass type I membrane protein that belongs to the Ephrin-B family of receptor tyrosine kinases that is involved in embryonic nervous and vascular system development. EPHB1/EPHT2 contains two fibronectin type-III domains, one protein kinase domain and one SAM (sterile alpha motif) domain. EPHB1 could stimulate fibroblast motility on extracellular matrix in a kinase-dependent manner, which also correlated with its association with Grb7, an adaptor molecule implicated in the regulation of cell migration. It binds to ephrin-B1, ephrin-B2 and ephrin-B3. EPHB1 plays an important roles in diverse biological processes including nervous system development, angiogenesis, and neural synapsis formation and maturation and may be involved in cell-cell interactions in the nervous system.
Function
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their self-renewal and reducing their activation and differentiation (By similarity).
Subcellular location
Cell membrane, Single-pass type I membrane protein, Early endosome membrane, Cell projection, dendrite
Protein Families
Protein kinase superfamily, Tyr protein kinase family, Ephrin receptor subfamily
Tissue Specificity
Preferentially expressed in brain.

Note: The presented information and documents (Manual, Product Datasheet and Safety Datasheet) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

Amount: 50ug
Available: In stock
available
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