Comparison

Recombinant Mouse 5'-nucleotidase (Nt5e), partial (Active)

Manufacturer Biomatik
Category
Type Proteins Recombinant
Specific against Mouse
Amount 50ug
Item no. BM-RPC26956-50ug
eClass 6.1 34160400
eClass 9.0 42020190
Available
Gene Name
Nt5e
Alternative Names
5'-nucleotidase, Ecto-5'-nucleotidase, CD73, 5'-NT
Uniprot
Q61503
Source
Mammalian cell
Expression Region
29-550aa
AA Sequence
WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGV ARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVY KGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPL LRNVKFPILSANIKARGPLAHQISGLFLPSKVLSV GGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQ PEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGV DIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADD GRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGN PILLNSSIPEDATIKADINQWRIKLDNYSTQELGR TIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHP DEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENL AAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQST GEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRV PIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELL KHDSGDQDISVVSEYISKMKVVYPAVEGRIKF
Sequence Info
Partial
Tag Info
C-terminal 6xHis-tagged
Theoretical MW
58.8 kDa
Purity
>95% as determined by SDS-PAGE.
Storage Buffer
0.2 um filtered 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5
Endotoxin Level
Less than 1.0 EU/ug as determined by LAL method.
Biological Activity
Specific activity as determined by its ability to hydrolyze the 5´-phosphate group from the substrate adenosine-5´-monophosphate (AMP) is greater than 10000 pmol/min/ug. The orthophosphate product is measured by a malachite green phosphate detection kit.
Shipping Condition
Ice packs
Storage
Short term: -20CC; Long term: -80CC. Minimize freeze and thaw cycles.
Expiry Date
1 year
Restriction
For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Relevance
Mouse CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity.CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
Function
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Subcellular location
Cell membrane, Lipid-anchor, GPI-anchor
Protein Families
5'-nucleotidase family

Note: The presented information and documents (Manual, Product Datasheet and Safety Datasheet) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

Amount: 50ug
Available: In stock
available
Questions about this Product?
 
Close