Anti-Protein argonaute-2 AGO2 Antibody

Primary Antibodies

97208 MW

WB: 1:500-1:1000
IHC: 1:50-1:200

Protein argonaute-2

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

Required for RNA-mediated gene silencing (RNAi) by the
RNA-induced silencing complex (RISC). The 'minimal RISC' appears
to include AGO2 bound to a short guide RNA such as a microRNA
(miRNA) or short interfering RNA (siRNA). These guide RNAs direct
RISC to complementary mRNAs that are targets for RISC-mediated
gene silencing. The precise mechanism of gene silencing depends on
the degree of complementarity between the miRNA or siRNA and its
target. Binding of RISC to a perfectly complementary mRNA
generally results in silencing due to endonucleolytic cleavage of
the mRNA specifically by AGO2. Binding of RISC to a partially
complementary mRNA results in silencing through inhibition of
translation, and this is independent of endonuclease activity. May
inhibit translation initiation by binding to the 7-methylguanosine
cap, thereby preventing the recruitment of the translation
initiation factor eIF4-E. May also inhibit translation initiation
via interaction with EIF6, which itself binds to the 60S ribosomal
subunit and prevents its association with the 40S ribosomal
subunit. The inhibition of translational initiation leads to the
accumulation of the affected mRNA in cytoplasmic processing bodies
(P-bodies), where mRNA degradation may subsequently occur. In some
cases RISC-mediated translational repression is also observed for
miRNAs that perfectly match the 3' untranslated region (3'-UTR).
Can also up-regulate the translation of specific mRNAs under
certain growth conditions. Binds to the AU element of the 3'-UTR
of the TNF (TNF-alpha) mRNA and up-regulates translation under
conditions of serum starvation. Also required for transcriptional
gene silencing (TGS), in which short RNAs known as antigene RNAs
or agRNAs direct the transcriptional repression of complementary
promoter regions.

Boster Bio Anti-Protein argonaute-2 AGO2 Antibody catalog # A00189. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.