Anti-SIRT2 Antibody

Primary Antibodies

43182 MW

ICC/IF: 1:50-1:200
FC: 1:50-1:100

NAD-dependent protein deacetylase sirtuin-2

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

NAD-dependent protein deacetylase, which deacetylates
internal lysines on histone and alpha-tubulin as well as many
other proteins such as key transcription factors (PubMed:24177535,
PubMed:12620231, PubMed:16648462, PubMed:18249187,
PubMed:18332217, PubMed:18995842, PubMed:20587414,
PubMed:21081649, PubMed:20543840, PubMed:22014574,
PubMed:21726808, PubMed:21949390, PubMed:22771473,
PubMed:23468428, PubMed:23908241, PubMed:24940000,
PubMed:24769394, PubMed:24681946). Participates in the modulation
of multiple and diverse biological processes such as cell cycle
control, genomic integrity, microtubule dynamics, cell
differentiation, metabolic networks, and autophagy. Plays a major
role in the control of cell cycle progression and genomic
stability. Functions in the antephase checkpoint preventing
precocious mitotic entry in response to microtubule stress agents,
and hence allowing proper inheritance of chromosomes. Positively
regulates the anaphase promoting complex/cyclosome (APC/C)
ubiquitin ligase complex activity by deacetylating CDC20 and FZR1,
then allowing progression through mitosis. Associates both with
chromatin at transcriptional start sites (TSSs) and enhancers of
active genes. Plays a role in cell cycle and chromatin compaction
through epigenetic modulation of the regulation of histone H4
'Lys-20' methylation (H4K20me1) during early mitosis. Specifically
deacetylates histone H4 at 'Lys-16' (H4K16ac) between the G2/M
transition and metaphase enabling H4K20me1 deposition by KMT5A
leading to ulterior levels of H4K20me2 and H4K20me3 deposition
throughout cell cycle, and mitotic S-phase progression
(PubMed:23468428). Deacetylates KMT5A modulating KMT5A chromatin
localization during the mitotic stress response (PubMed:23468428).
Deacetylates also histone H3 at 'Lys-57' (H3K56ac) during the
mitotic G2/M transition. Upon bacterium Listeria monocytogenes
infection, deacetylates 'Lys-18' of histone H3 in a receptor
tyrosine kinase MET- and PI3K/Akt-dependent manner, thereby
inhibiting transcriptional activity and promoting late stages of
listeria infection (PubMed:23908241). During oocyte meiosis
progression, may deacetylate histone H4 at 'Lys-16' (H4K16ac) and
alpha-tubulin, regulating spindle assembly and chromosome
alignment by influencing microtubule dynamics and kinetochore
function. Deacetylates histone H4 at 'Lys-16' (H4K16ac) at the
VEGFA promoter and thereby contributes to regulate expression of
VEGFA, a key regulator of angiogenesis (PubMed:24940000).
Deacetylates alpha-tubulin at 'Lys-40' and hence controls neuronal
motility, oligodendroglial cell arbor projection processes and
proliferation of non-neuronal cells. Phosphorylation at Ser-368 by
a G1/S-specific cyclin E-CDK2 complex inactivates SIRT2-mediated
alpha-tubulin deacetylation, negatively regulating cell adhesion,
cell migration and neurite outgrowth during neuronal
differentiation. Deacetylates PARD3 and participates in the
regulation of Schwann cell peripheral myelination formation during
early postnatal development and during postinjury remyelination.
Involved in several cellular metabolic pathways. Plays a role in
the regulation of blood glucose homeostasis by deacetylating and
stabilizing phosphoenolpyruvate carboxykinase PCK1 activity in
response to low nutrient availability. Acts as a key regulator in
the pentose phosphate pathway (PPP) by deacetylating and
activating the glucose-6-phosphate G6PD enzyme, and therefore,
stimulates the production of cytosolic NADPH to counteract
oxidative damage. Maintains energy homeostasis in response to
nutrient deprivation as well as energy expenditure by inhibiting
adipogenesis and promoting lipolysis. Attenuates adipocyte
differentiation by deacetylating and promoting FOXO1 interaction
to PPARG and subsequent repression of PPARG-dependent
transcriptional activity. Plays a role in the regulation of
lysosome-mediated degradation of protein aggregates by autophagy
in neuronal cells. Deacetylates FOXO1 in response to oxidative
stress or serum deprivation, thereby negatively regulating FOXO1-
mediated autophagy (PubMed:20543840). Deacetylates a broad range
of transcription factors and co-regulators regulating target gene
expression. Deacetylates transcriptional factor FOXO3 stimulating
the ubiquitin ligase SCF (SKP2)-mediated FOXO3 ubiquitination and
degradation (By similarity). Deacetylates HIF1A and therefore
promotes HIF1A degradation and inhibition of HIF1A transcriptional
activity in tumor cells in response to hypoxia (PubMed:24681946).
Deacetylates RELA in the cytoplasm inhibiting NF-kappaB-dependent
transcription activation upon TNF-alpha stimulation. Inhibits
transcriptional activation by deacetylating p53/TP53 and EP300
(PubMed:18249187). Deacetylates also EIF5A (PubMed:22771473).
Functions as a negative regulator on oxidative stress-tolerance in
response to anoxia-reoxygenation conditions. Plays a role as tumor
suppressor (PubMed:22014574).

Boster Bio Anti-SIRT2 Antibody catalog # A00433. Tested in ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.